低氧模拟剂氯化钴对胃癌细胞BGC823中S100A4基因表达的影响  

Effect of cobalt chloride on S100A4 expression in human gastric cancer cell BGC823

作　　者：滑君[1] 付浩[1,2] 张瑞秀[1] 陈丹琦[1] 闫扬[1] 陈芳杰[1] 孙开来[1] 孙秀菊[1] 

机构地区：[1]中国医科大学医学遗传学教研室,沈阳110001 [2]沈阳医学院生物化学教研室,沈阳110046

出　　处：《遗传》2008年第12期1563-1566,共4页Hereditas（Beijing)

基　　金：国家自然科学基金项目(编号:30570848)资助~~

摘　　要：S100A4基因是肿瘤侵袭转移相关的重要基因,该基因高表达与胃癌浸润、淋巴结转移及胃癌细胞体外侵袭力密切相关。为探讨S100A4基因高表达的机制,文章应用低氧模拟剂氯化钴处理胃癌细胞BGC823,RT-PCR、免疫组化、免疫荧光及Westernblotting方法分别检测BGC823细胞中S100A4mRNA及蛋白表达情况。结果显示,氯化钴处理胃癌BGC823细胞后,S100A4mRNA及蛋白表达明显增加。提示低氧模拟剂氯化钴可促进胃癌细胞BGC823中S100A4基因表达。S100A4 is an important metastasis-associated gene. Researches have confirmed the close correlation between overexpression of S100A4 gene and gastric cancer＇s infiltration, lymph node metastasis and in vitro invasiveness of gastric cancer cells. In order to investigate the mechanism of overexpression of S100A4 gene, hypoxia mimetic cobalt chloride （COC12） was used to treat gastric cancer cell BGC823, and then the expression of S100A4 mRNA and protein in BGC823 cells were detected by RT-PCR, immunohlstochemistry, immunofluroescence, and Western blotting analysis. After treat- ment with COC12, the expression of S100A4 mRNA and protein in BGC823 cell was increased. These results suggested that hypoxia mimetic cobalt chloride could increase the expression of S100A4 gene in gastric cancer cell BGC823.

关 键 词：胃癌 低氧 氯化钴 S100A4基因 

分 类 号：R735.2[医药卫生—肿瘤]