膀胱肿瘤抗原致敏的树突状细胞诱导T淋巴细胞抗肿瘤效应的研究  

Anti-tumor effect of T lymphocytes induced by dendritic cells pulsed with bladder tumor antigen

作　　者：王江平[1] 王勤章[1] 丁国富[1] 闫东[1] 钱彪[1] 

机构地区：[1]石河子大学医学院第一附属医院泌尿外科,新疆石河子832000

出　　处：《现代肿瘤医学》2008年第12期2061-2063,共3页Journal of Modern Oncology

基　　金：新疆生产建设兵团医药卫生专项资助课题(No.NKB03SDXYY30SY)

摘　　要：目的:研究经膀胱肿瘤抗原致敏后的树突状细胞(DC)对T淋巴细胞的激活、增殖作用及对T24膀胱肿瘤细胞的抑癌效应。方法:分离健康供血者外周血单个核细胞及T淋巴细胞,联合应用粒/巨噬细胞集落刺激因子(GM-CSF)及白介素-4(IL-4)从单个核细胞中培养出DC,以人膀胱癌细胞系T24肿瘤细胞裂解物刺激DC,检测经膀胱肿瘤抗原致敏后的DC对T淋巴细胞的细胞增殖动力学影响并用MTT显色法测定致敏DC诱导的T淋巴细胞对T24膀胱肿瘤细胞体外的抗肿瘤效应。结果:膀胱癌细胞裂解物致敏的DC可诱导T淋巴细胞强烈的增殖反应,与对照组比较具有显著性差异(P<0.01);增殖后的T淋巴细胞对T24膀胱肿瘤细胞有明显的细胞毒作用。结论:经膀胱肿瘤抗原致敏的DC能诱导产生显著的T淋巴细胞增殖,在体外有明显的抑癌效应。Objective：To investigate the proliferation of cytotoxic T lymphocytes （CTL） induced by dendritic cells pulsed with bladder tumor antigen and anti - tumor effects on T24 bladder carcinoma cells in vitro. Methods： Isola- ted and purified DC derived from with granulocyte/macrophage colony stimulating factor and interleukin 4 ; extracted tumor -associated antigen （TAA） form human bladder cancer line T24 tumor cells; Stimulated T lymphocytes with DC pulsed by TAA to produce CTL; Proliferation and cytotoxic effect of CTL on bladder carcinoma cells were meas- ured by liquid scintillation counter and MTT assays. Results： Cytotoxic T cell had begun proliferation stimulated by DC. Peak proliferation was observed, P 〈 0.01 as compare with medium. CTL had highly cytotoxicity against bladder carcinoma cells in vitro. Conclusion： DC pulsed by TAA form human bladder cancer could induce higher cytotoxic T lymphocyte immune response producing greater effects on suppressing bladder - tumor cells growth in vitro .

关 键 词：肿瘤抗原 树突状细胞 膀胱肿瘤 T淋巴细胞 细胞毒性 

分 类 号：R734.14[医药卫生—肿瘤]