辛伐他汀对SH-SY5Y神经细胞中胆碱能神经系统的刺激作用  

Stimulation of Simivastatin on Cholinergic Nerve System in SH-SY5Y Cells

作　　者：官志忠[1,2] 田茂萍 肖雁[1,2] 

机构地区：[1]贵阳医学院病理学教研室,贵州贵阳550004 [2]贵阳医学院分子生物学重点实验室,贵州贵阳550004 [3]贵阳市妇幼保健院,贵州贵阳550002

出　　处：《贵阳医学院学报》2008年第6期573-576,共4页Journal of Guiyang Medical College

基　　金：国家自然科学基金资助项目(30870986);科技部国际合作基金资助项目(2006DFA3353)

摘　　要：目的:研究胆固醇降解药辛伐他汀(Simivastatin)对SH-SY5Y神经细胞中胆碱能神经的刺激作用,探讨史他汀类药物在神经退变性疾病中的神经性保护机制。方法:体外培养SH-SY5Y神经细胞(源于人神经母细胞瘤细胞),在培养液中加入辛伐他汀培养48h,然后用比色法测定细胞MTT还原率及胆碱酯酶活性,用蛋白印迹方法测定尼古丁受体α7和α3蛋白水平,RT-PCR方法测定该类受体mRNA表达水平。结果:辛伐他汀能降低培养细胞中乙酰胆碱酯酶和丁酰胆碱酯酶活性(分别降低41.9%和18.1%);增强尼古丁受体α7亚单位的蛋白和mRNA表达水平(分别升高23%和32%),但对α3无明显影响。结论:辛伐他汀可以刺激胆碱能神经系统,可能具有神经性保护作用。Objective： To investigate the effect of simivastatin on cholinergic nerve system and to understand neuroprotective mechanisms of the drug in neurodegenerative diseases. Methods： SH-SYSY cells （human neuroblastoma cell line） , were incubated with simivastatin for 48 hr. The MTT reduction and cholinesterase activity were measured by using photometric method; the expressions of nicotinic acetylcholine receptor （nAChR）subunits α7 and α3 at protein and mRNA levels were detected with Western blotting and RT-PCR techniques respectively. Results： Simivastatin inhibited the activities of acetylcholinesterase and butyrylcholinesterase by 41.9% and 18.1%, respectively, in the cultured cells. The drug increased the expressions of nAChR ct7 at both protein and mRNA levels by 23% and 32%, respectively; however, it dose not induce any changes in the expression of nAChR α3. Conclusions： Simivastatin can stimulate the cholinergic nervous system and may play certain neuroprotective effects.

关 键 词：辛伐他汀 胆碱酯酶类 尼古丁受体 SH-SYSY神经母细胞瘤细胞 

分 类 号：R749.16[医药卫生—神经病学与精神病学]