水貂株犬瘟热病毒融合蛋白基因的克隆与序列分析  

Cloning and Sequence Analysis of Fusion Protein Gene of Canine Distemper Virus Isolated from Mink

作　　者：苏凤艳[1] 宗春苗[1] 王卓聪[1] 丁庆东[1] 王全凯[1] 

机构地区：[1]吉林农业大学中药材学院,吉林长春130118

出　　处：《西北农业学报》2008年第6期21-24,共4页Acta Agriculturae Boreali-occidentalis Sinica

摘　　要：以犬瘟热病毒水貂分离株RNA为模板,经RT-PCR反应,扩增出融合蛋白基因的1053bp DNA片段,通过T-A克隆技术,成功构建克隆载体PMD-18T/F。序列分析表明:分离株与01-2689株、2544-Han95株、A75-17株、DOGDK91C株、00-2601株、ONP株、PDV-2株核苷酸序列同源性分别为94.3%、93.5%、94.4%、93.6%9、5.3%、97.3%和94.5%;氨基酸序列同源性分别为97.2%、96.6%、97.4%、97.4%、97.4%、97.2%和98.0%。抗原指数分析表明分离株与疫苗ONP株、强毒A75-17株在40-50位氨基酸间存在明显差异。The genomic RNA was extracted from isolated strain. The 1053bp DNA segment of fusion protein（F） gene was amplified by reverse transcriptase polymerase chain reaction （RT-PCR）. The clone vector PMD 18T-CDVSJ F was constructed successfully by T-A clone technique. The sequence of the fusion gene and deduced amino acid of isolated strain showed an identity of 94.3%, 93.5 %, 94.4%, 93.6%, 95.3%, 97.3%, 94.5% and 97.2%, 96.6%, 97.4%, 97.4%, 97.4%, 97.2%, 98.0% with 01--2689, 2544Han95, A75--17, DOGDK91C, 00--2601, ONP, PDV-2,respectively. Antigenicity analysis showed that isolated strain was different from ONP and A75-17 in 40-50th amino acid antigenicity.

关 键 词：水貂 犬瘟热病毒 融合蛋白基因 克隆 序列分析 

分 类 号：S852.65[农业科学—基础兽医学]