PNMA5基因在肝癌组织中的表达及功能研究  

Expression of PNMA5 in hepatocellular carcinoma tissues and its function

作　　者：滕小梅[1,2,3] 邓庆[1,2] 韩泽广[1,2] 黄健 

机构地区：[1]上海交通大学附属瑞金医院上海血液学研究所,上海200025 [2]上海市疾病与健康基因组学实验室-省部共建国家重点实验室培育基地,国家人类基因组南方研究中心,上海201203 [3]苏州大学附属第一人民医院心血管外科,苏州215006

出　　处：《肿瘤》2008年第11期911-915,共5页Tumor

基　　金：国家"九七三"项目(编号:2006CB910402);上海市科委资助项目(编号:.06ZR14069)

摘　　要：目的:研究功能未知基因PNMA5在肝癌组织中的表达及其与肝癌细胞生长的关系;方法:利用肝癌基因表达谱数据研究发现PNMA5基因在肝癌组织中高表达。采用RT-PCR及实时荧光定量-PCR(real-time fluorescemt quantitative PCR,RFQ-PCR)方法检测PNMA5在人类正常组织以及肝癌组织和癌旁组织中的表达水平;利用RNA干扰技术沉默肝癌细胞株(Focus和PLC)内PNMA5基因的表达;CCK-8实验定量分析内源性PNMA5基因被沉默前后的Focus和PLC细胞生长曲线的变化。结果:在14种人正常组织中,PNMA5只在睾丸、卵巢和脑组织中高表达,PNMA5基因在42%(5/12)的肝癌组织中有明显的上调(与癌旁组织比大于2倍以上,P<0.05)。沉默肝癌细胞株Focus和PLC中内源性PNMA5基因表达后,2种肝癌细胞的生长均受到明显的抑制(P<0.01),同时,从G1期进入S期细胞明显减少(P<0.01)。结论:PNMA5在维持肝癌细胞恶性表型过程中可能起到重要的作用,可能是一个新的肿瘤-睾丸相关基因。进一步研究其功能有可能发现肝癌发病的新机制,成为肝癌治疗的新靶点。Objective ： To study the expression of function-unknown PNMA5 gene in hepatocellular carcinoma （HCC） and its relationship with proliferation of HCC. Methods： PNMA5 was over-expressed in HCC analyzed by gene expression profiling. We investigated the expression of PNMA5 through RT-PCR and real-time fluorescent quantitative PCR （RFQ-PCR） in normal liver tissues, HCC, and adjacent non-cancerous liver tissues （non-HCC）. PNMA5 gene was silenced in Focus and PLC liver cancer cells by using RNA interference technology. The changes in cell growth curve were quantitatively analyzed by performing CCK-8 experiment after silencing the endogenous PNMA5 gene expression. Results： PNMA5 was mainly expressed in testis, ovary, and brain tissues out of the 14 kinds of normal human tissues. PNMA5 mRNA was significantly upegulated in 42% （5/12） HCC specimens, which was 2-fold higher than that in non-HCCs （ P 〈 0.05 ）. Interestingly, the growth of Focus and PLC cells was markedly inhibited after silencing PNMA5 gene expression （ P 〈 0.01 ）, and the number of tumor cells progressing through G1 and entering S phase was significantly decreased as compared with the control cells transfected with siRNA-NC （P 〈 0.01 ）. Conclusion： PNMA5 plays an important role in maintaining the malignant phenotype of HCC. It may be a novel tumor-testis associated gene. Further investigation of its function may help to find the new mechanism for the HCC tumorigenesis. PNMA5 may become a new candidate target in HCC treatment.

关 键 词：肝肿瘤 实验性 RNA 小分子干扰 基因 PNMA5 

分 类 号：R735.7[医药卫生—肿瘤]