优福定节拍性化疗可抑制MKN-45胃癌的血管生成拟态  

Metronomic chemotherapy with UFT inhibits vasculogenic mimicry formations in human MKN-45 gastric cancer cells

作　　者：吴欣[1] 闫明霞[2] 于观贞[1] 王喜[1] 陈颖[3] 姚明[2] 王杰军[1] 

机构地区：[1]第二军医大学长征医院肿瘤科,上海200070 [2]上海市肿瘤研究所实验病理研究室,上海200032 [3]第二军医大学长海医院病理科,上海200433

出　　处：《肿瘤》2008年第11期929-932,共4页Tumor

摘　　要：目的:研究优福定(tegafur/uracil,UFT)节拍性化疗对MKN-45荷瘤裸鼠血管生成拟态(vasculogenic mimicry,VM)的影响,探索其可能机制。方法:建立荷瘤动物模型,UFT对荷瘤动物进行节拍性治疗,观察其疗效及不良反应;HE、过碘酸-雪夫(periodic acid Schiff,PAS)染色法检测VM,免疫组织化学检测CD31的表达。结果:MKN-45肿瘤细胞在活体内可表达CD31内皮相关分子,形成PAS染色阳性、类血管生成的图形结构。UFT节拍性化疗抑制VM形成,而最大耐受剂量UFT却没有此抑制作用。结论:UFT节拍性化疗可抑制MKN45胃癌的形成。Objective ： To examine the effects of metronomic chemotherapy with UFT on vasculogenic mimicry （VM） formations in nude mice bearing MKN-45 gastric cancer cells and explore the possible mechanism. Methods： The tumor-bearing mouse model was established. UFT-based metronomic chemotherapy was performed. The efficacy and adverse reaction were observed. The number of VM was counted by HE and periodic acid Schiff （PAS） staining. The expression of CD31 was analyzed by immunohistochemistry. Results： MKN-45 tumor cells expressed CD3 endothelium-related molecules and formed PAS-positive vasculogenic mimicry. UFT-based metronomic chemotherapy markedly inhibited the formation of VM. But UFT at maximum using dosage had no inhibitory effects on VM formations. Conclusion： Metronomic UFT chemotherapy inhibits VM formations by MKN-45 gastric cancer cells.

关 键 词：胃肿瘤 新生血管化 病理性 药物疗法 异种移植模型抗肿瘤试验 

分 类 号：R735.7[医药卫生—肿瘤]