多器官功能障碍综合征肺IL-10的变化对树突细胞功能的影响  

Influence of lung IL-10 on dendritic cells in multiple organs dysfunction syndrome

作　　者：王宏伟[1] 陆江阳[1] 田光[1] 杨毅[1] 李韶然[1] 

机构地区：[1]解放军总医院第一附属医院病理科,北京100037

出　　处：《解放军医学杂志》2008年第12期1421-1423,共3页Medical Journal of Chinese People's Liberation Army

基　　金：全军医学科研"十一五"规划面上项目资助课题(06MB307)

摘　　要：目的探讨多器官功能障碍综合征（MODS）模型小鼠肺IL-10对树突状细胞功能的影响及其在免疫紊乱中的作用与意义。方法采用腹腔注射酵母多糖法建立小鼠MODS模型。实验分为对照组与致伤后3-6h、12-48h、120-168h和240-288h组（实验组）。采用RT-PCR检测肺组织中CCR7 mRNA的表达。免疫组化标记检测肺趋化因子受体CCR7与IL-10的表达。流式细胞术检测外周血MHC-Ⅱ阳性的单个核细胞数量。结果与对照组比较,3-6h组肺内IL-10标记阳性细胞数和CCR7水平升高,外周血MHC-Ⅱ阳性的单个核细胞数量减少。12-48h组肺IL-10标记阳性细胞数和CCR7表达水平显著升高,外周血MHC-Ⅱ阳性的单个核细胞减少。240-288h组IL-10较对照组升高非常显著,CCR7表达水平较12-48h组明显下降,外周血MHC-Ⅱ阳性的单个核细胞数下降至最低点。结论MODS发生时,肺IL-10表达水平的升高一方面可以抑制由树突状细胞诱导的过度免疫损伤；另一方面,也可能通过抑制树突状细胞表达CCR7和降低外周血MHC-Ⅱ阳性的抗原提呈细胞数量最终诱导免疫麻痹的发生。Objective To explore the influence of lung IL-10 on interstitial dendritic cells in multiple organ dysfunction syndrome （MODS）, and its role in immunological dysfunction. Methods The model of MODS was replicated by injecting zymosan into the peritoneal cavity of C57BL/6 mice. The mice were then randomly divided into normal groups as well as the groups of 3-6 hours, 12-48 hours, 5-7 days and 10-12 days post trauma. The level of CCR7 mRNA in lung was detected by RT-PCR and the proteins of CCR7 and IL-10 were immunohistochemically stained. The number of mononuclear cells in MHC-Ⅱ positive peripheral blood was determined by flow cytometry. Results At the early stage of injury （3-6 hours）, the number of IL-10 positive cells and the expression of CCR7 increased, but the number of mononuclear cells in MHC-Ⅱ positive peripheral blood decreased. At the stage of 12-48 hours, the number of IL-10 positive cells and the expression of CCR7 continued to increase obviously, and that of mononuclear cells in MHC-Ⅱ positive peripheral blood went on decreasing. At the stage of 10-12 days, the number of IL-10 positive cells increased dramatically compared with that in normal group, but the expression of CCR7 declined obviously in contrast to that in 12-48 hours, and the number of monunuclear cells in MHC-Ⅱ positive peripheral blood decreased to the lowest level. Condusions The expression of IL-10 in lung could inhibit the excessive immunological damage induced by dendritic cells on the one hand, and it probably leads to immunosuppression by reducing the CCR7 expression of dendritic cells and the number of mononuclear cells in MHC-Ⅱ positive peripheral blood on the other hand.

关 键 词：多器官功能衰竭 白细胞介素10 树突细胞 受体 CCR7 MHC-Ⅱ分子 

分 类 号：R365[医药卫生—病理学]