Bmi-1、Ki67在大肠肿瘤组织中的表达及其意义  

Expression and Significance of Bmi-1 and Ki67 in Colorectal Carcinoma Tissues

作　　者：林妙霞[1] 文卓夫[1] 冯智英[2] 何丹[2] 

机构地区：[1]中山大学附属第三医院消化内科,广东广州510630 [2]中山大学附属第三医院病理科,广东广州510630

出　　处：《癌症》2008年第12期1321-1326,共6页Chinese Journal of Cancer

基　　金：广东省科技攻关项目(No.2006B35502009)~~

摘　　要：背景与目的:多梳基因家族成员的Bmi-1基因被认为是一种癌基因,在多种肿瘤组织中均有表达,而增殖细胞核抗原Ki67是反映细胞增殖程度的重要参考指标。本研究旨在通过检测大肠肿瘤组织中Bmi-1、Ki67蛋白表达情况及意义,并探讨两者在大肠癌中的表达的相关性。方法:采用免疫组织化学方法检测Bmi-1、Ki67蛋白在60例大肠癌、30例大肠腺瘤及20例正常大肠粘膜组织中的表达情况及其与大肠癌临床病理特征及患者生存率的关系,并探讨大肠癌中Bmi-1蛋白表达与Ki67蛋白的相关性。结果:Bmi-1蛋白在大肠癌、大肠腺瘤及正常肠粘膜组织中高表达率分别为25.0%、6.7%、0%,而Ki67蛋白的高表达率分别为18.3%、3.3%、0%;Bmi-1、Ki67蛋白在大肠癌中表达明显高于腺瘤组及正常组(P<0.05);采用卡方检验显示,Bmi-1蛋白高表达与有无远处转移密切相关(P<0.01),而与TNM分期有关(P<0.05)。采用Logistic回归分析显示只有远处转移与Bmi-1蛋白高表达有关(P<0.01,OR>1)。卡方检验显示,Ki67蛋白高表达与患者年龄、有无远处转移及TNM分期密切相关(P<0.05),Logistic回归分析显示只有患者年龄与Ki67蛋白高表达有关(P<0.05,OR<1);Kaplan-Meier生存分析显示Bmi-1、Ki67蛋白高表达患者生存率明显低于低表达患者(P<0.05);大肠癌组织中Bmi-1蛋白表达与Ki67蛋白表达相关性无统计学意义(r=0.224,P>0.05)。结论:Bmi-1、Ki67蛋白表达与大肠癌的发生、转移及预后关系密切,可作为评估患者浸润转移及预后的参考指标;Bmi-1蛋白对Ki67蛋白表达可能有间接的调控作用。BACKGROUD ＆ OBJECTIVE. Bmi-1, a putative oncogene, is a member of the polycomb group genes, and is expressed in many human tumors. Ki67 is an important nuclear antigen associated with cell proliferation. This study was to investigate the expression, significance and correlation of Bmi-1 and Ki67 in colorectal carcinoma （CRC） tissues. METHODS： Protein expressions of Bmi-1 and Ki67 in sixty CRC, 30 adenomas and 20 normal colorectal mucosal tissues were detected using immunohistochemistry. Correlations of the expression of Bmi-1 and Ki67 to clinicopathological features and survival of patients were analyzed. RESULTS： The expression rates of Bmi-1 and Ki67 were 25.0%, 6.7%, 0% and 18.3%, 3.3% and 0% in CRC, adenomas and normal colorectal mucosal tissues, respectively. The expression of Bim-1 and Ki67 were significantly higher in CRC than in adenomas and normal colorectal mucosal tissues （P〈0.05）. Chi-square test revealed that the overexpression of Bmi-1 protein in CRC was correlated to distant metastasis （P〈0.01） and TNM stage （P〈0.05）, while the overexpression of Ki67 protein was associated with age, distant metastasis and TNM stage （P〈0.05）. Further logistic regression analysis showed that only distant metastasis was correlated with Bmi-1 overexpression （P〈0.01, OR〉1）, and only age was correlated with Ki67 overexpression（P〈0.05, OR〈 1）. Kaplan-Meier survival analysis showed that the survival was significantly shorter in CRC patients with overexpression than low expression of Bmi-1 and Ki67 （P〈0.05）. CONCLUSIONS： Overexpression of Bmi-1 and Ki67 protein are significantly correlated with tumorigenesis, metastasis and prognosis of CRC. Bmi-1 and Ki67 might be used to predict the prognosis of CRC. Bmi-1 might regulate the expression of Ki67 indirectly in CRC patients.

关 键 词：BMI-1蛋白 KI67蛋白 大肠肿瘤 免疫组织化学 

分 类 号：R735.34[医药卫生—肿瘤]