DNA聚合酶Polι在紫外损伤诱导基因组高突变中的作用  

DNA Polymeraseι Contributes to UV-damage Induced Hypermutagenesis

作　　者：曾益军[1] 周虎传[1] 位全芳[1] 赵谦[1] 杨劲[1] 

机构地区：[1]第三军医大学基础部生物化学与分子生物学教研室,重庆市400038

出　　处：《医学分子生物学杂志》2008年第6期484-487,共4页Journal of Medical Molecular Biology

基　　金：国家自然科学基金( No.30770460);全军医学科研"十一五"计划国际合作项目(No.06H026)~~

摘　　要：目的研究DNA聚合酶Polι在紫外损伤耐受及基因组高突变产生中的生物学作用。方法紫外线照射稳定高表达DNA聚合酶iota(DNA polymerase iota,Polι)的HEK293T细胞系(Polι-HEK293T),MTT法检测紫外损伤后细胞的存活率;supF报告基因体内突变检测系统检测基因的突变频率。结果MTT法结果提示紫外照射后稳定高表达Polι的细胞系与对照组相比细胞存活率没有显著差异;supF报告基因结果显示稳定高表达Polι的细胞系其基因突变频率高于对照组细胞。结论DNA聚合酶Polι的高表达对紫外损伤后细胞的存活率没有显著影响,不能提高细胞对紫外损伤的耐受,在紫外损伤中polι主要参与诱发体内基因组高突变的产生。Objective To study the function of DNA Polymerase iota （DNA Polymerase iota, Pol ） in UV-induced DNA damage response in HEK293T cells. Methods The HEK293T cells stably over-expressing Pol were irradiated with different dose of UV light. The cell viability was detected with MTF assay. The frequency of mutation was investigated with in vivo supF mutation detection system. Results After exposed to UV, Pol 1 stably transfected cells showed no difference in survival rate compared to non-exposed control, whereas the mutation frequency of supF was higher than that of the control. Conclusions Pol does not affect the UV-sensitivity of HEK293T cells, but contributes to the UV-damage induced mutagenesis.

关 键 词：DNA聚合酶iota HEK-293T细胞 DNA损伤应答 突变 

分 类 号：Q786[生物学—分子生物学]