人胰腺癌荷瘤裸鼠异位模型建立  

Establishment of heterotopic model with pancreatic cancer in nude mice

作　　者：陈启龙[1] 孙卫红[2] 陈占峰[1,2] 

机构地区：[1]新疆医科大学第一附属医院普通外科,新疆乌鲁木齐830011 [2]日照市人民医院普通外科,山东日照276826

出　　处：《新疆医科大学学报》2008年第10期1373-1375,共3页Journal of Xinjiang Medical University

摘　　要：目的:用人体胰腺癌细胞株,建立人胰腺癌荷瘤裸鼠异位模型。方法:裸鼠在SPF洁净环境下饲养,将4×106人胰腺癌细胞株SW1990肿瘤细胞悬液0.2 ml注射于裸鼠背部皮下,建立肿瘤模型。结果:肿瘤细胞移植成功率为90%(54/60),生长迅速,移植瘤灰白色,质硬。组织学检查符合胰腺低分化腺癌。结论:异位裸鼠人胰腺癌移植瘤模型易复制,时间短,成功率高,为胰腺癌体内研究提供了理想的动物模型。Objective. To establish heterotopic model with pancreatic cancer in nude mice through the direct injection of human pancreatic cancer cells. Methods： The nude mice were housed and maintained in laminar flow cabinets under specific pathogen-free conditions. A tumor cell suspension （SW1990） of 4×10^6 cells in 0.2 ml RPMI1640 was injected into the subcutaneous dorsa along the proximal midline in the mice. Resuits： The pancreatic masses presented in 90% （54/60） nude mice after injection of human pancreatic cancer cells. The masses developed very quickly, were usually firm and white, and were low grade differential adenocarcinomar histologically. Conclusions： Establishment of heterotopic model with pancreatic cancer in nude mice was relatively esay, quickly, and especially with high successful rate. So it can be considered as an ideal animal model for study on panceatic cancer in vivo.

关 键 词：胰腺癌 裸鼠 动物模型 

分 类 号：R-332[医药卫生] R735.9