gyrA和parC介导的淋病奈瑟菌对环丙沙星耐药机制研究  

A research of ciprofloxacin resistance and mechanism in gyrA and parC induced Neisseria gonorrhoeae

作　　者：陈鹏[1] 蔡玲[1] 夏威为[1] 陈建国[1] 

机构地区：[1]江苏大学附属人民医院,江苏省镇江市第一人民医院,江苏镇江212002

出　　处：《右江民族医学院学报》2008年第6期937-939,共3页Journal of Youjiang Medical University for Nationalities

基　　金：江苏省镇江市社会发展项目(FZ2006065)

摘　　要：目的探讨淋病奈瑟菌对环丙沙星的耐药机制。方法采用K-B纸片法检测27株淋病奈瑟菌的耐药率,PCR扩增gyrA和parC基因,测序分析DNA序列。结果27株淋病奈瑟菌对青霉素、四环素以及环丙沙星的耐药率均为100%,而大观霉素和头孢曲松100%敏感;DNA序列分析表明:环丙沙星耐药株均存在gyrA和parC基因的突变,其中gyrA基因的突变位点发生在第91位、95位氨基酸,parC基因的突变发生在第86位、87位、88位和91位氨基酸。结论淋病奈瑟菌的耐药与gyrA和parC基因突变有关。Objective To explore the resistance and mechanisril of Neisseria gonorrhoeae to ciprofloxacin. Methods A K- B method was used to determine the resistance rate of 27 strains of Neisseria gonorrhoeae. A PCR technique was utilized to detect gyrA and parC genes followed by analyzing DNA sequence. Results 27 strains of Neisseria gonorrhoeae demonstrated 100 % resistance to Penicillin, tetracycline and ciprofloxacin. While none of the strains appeared to be resistant to spectinomycin and ceftriaxone. In the ciprofloxacin resistance strain, the DNA sequenee analysis confirmed mutations in gyrA at 91 and 95. Mutations in parC were at 86, 87, 88 and 91.Conclusion Mutations of gyrA and parC genes had contributed to the development of Fluoroquinolone resistance.

关 键 词：奈瑟球菌 淋病 环丙沙星 抗药性 GYRA基因 PARC基因 

分 类 号：R915.1[医药卫生—微生物与生化药学]