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机构地区:[1]北华大学生命科学研究中心,吉林吉林132013 [2]齐齐哈尔医学院药物研究所,黑龙江齐齐哈尔161042
出 处:《北华大学学报(自然科学版)》2008年第6期510-512,共3页Journal of Beihua University(Natural Science)
基 金:吉林省教育厅科研基金项目(2006-87)
摘 要:目的确定人表皮生长因子受体-2(HER-2)特异性锤头状核酶(hammerhead ribozyme,RZ)对雌激素受体阴性乳腺癌细胞MDA-MB-453的影响.方法应用RT-PCR、免疫细胞化学检测RZ对MDA-MB-453细胞HER-2表达的影响;MTT法检测RZ对细胞增殖的影响.结果RZ转染入MDA-MB-453细胞中,RT-PCR检测出转染RZ的MDA-MB-453细胞中HER-2 mRNA表达量明显下降;免疫细胞化学方法检测细胞内的HER-2蛋白表达下降;MTT法检测细胞增殖活性明显受抑.结论RZ在MDA-MB-453细胞内有效抑制靶基因HER-2mRNA和蛋白的表达,同时可抑制细胞增殖,有助于进一步研究雌激素受体阴性乳腺癌细胞HER-2信号转导通路.Objective To investigate the effect of hammerhead ribozyme targeting of HER-2 mRNA on estrogen receptor negative breast cancer cells MDA-MB-453. Methods The changes of HER-2 mRNA in MDA-MB-453 cells were detected by RT-PCR. Then,the effect on the expression of HER-2 protein was detected by immunocytochemistry(ICC). The cellular proliferation of MDA-MB-453 cell was tested by MTT assay. Results The expression of HER-2 mRNA in MDA-MB-453 cells transfected ribozyme which detected by RT-PCR significantly decreased. ICC showed that the expression of HER-2 protein was inhibited. MTT assay showed that the proliferation of MDA-MB-453 cell was inhibited. Conclusion RZ effectively inhibited the expression of HER-2 gene and protein in MDA-MB-453 cells,also inhibited proliferation of MDA-MB-453 cells. It helps to study deeply the HER-2 signal transduction pathway.
关 键 词:人表皮生长因子受体2(HER-2) 锤头状核酶 雌激素受体阴性乳腺癌 基因治疗
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