凋亡相关蛋白Livin及Caspase-3在膀胱移行细胞癌中的表达及临床意义  

Expression of Livin and Caspase-3 in Transitional Cell Carcinoma of Bladder and Their Clinical Significance

作　　者：祝兴旺[1] 宋彦[1] 张明明[2] 宋永胜[2] 

机构地区：[1]中国医科大学附属盛京医院泌尿外科,辽宁沈阳110004 [2]中国医科大学附属盛京医院病理科,辽宁沈阳110004

出　　处：《医学临床研究》2008年第11期1955-1957,1960,共4页Journal of Clinical Research

摘　　要：【目的】探讨凋亡相关蛋白Livin及Caspase-3在膀胱移形细胞癌(BTCC)中的表达及其临床意义。【方法】应用SP免疫组织化学法检测55例膀胱移行细胞癌及10例正常膀胱黏膜组织石蜡切片中Livin和Caspase-3表达的情况,结合临床资料进行分析,并探讨两者相关性。【结果】Livin在膀胱移形细胞癌标本中的表达阳性率为76.4%(42/55),而正常对照组中无一例呈阳性表达;Caspase-3在膀胱移行细胞癌标本中的表达阳性率为32.7%(18/55),与对照组阳性率90%(9/10)相比差异有统计学意义(P<0.05)。Livin的表达与膀胱移行细胞癌的初发和复发显著相关(P<0.05),但与组织学分级、临床病理分期、转移与否、肿瘤数目无关;Caspase-3的表达与膀胱移行细胞癌的初发复发、组织学分级、临床病理分期、转移与否、肿瘤数目均无关。相关性分析表明,膀胱移行细胞癌中的Livin表达与Caspase-3表达呈负相关。【结论】Livin蛋白与膀胱癌的发生和复发有关,可作为BTCC早期诊断和评判术后高复发风险的一项重要指标,且Livin的高表达和Caspase-3蛋白的低表达在膀胱移行细胞癌的发生发展起着重要作用。[Objective]To investigate the expression of livin and caspase-3 in bladder transitional cell carcinoma（BTCC） and their clinical significance.[Methods]The expressions of livin and caspase-3 were detected by streptavidin-peroxidase immunohistochemical method in the paraffin embedded sections of 55 BTCC patients,and their clinical data were analyzed,and their correlation was discussed.Normal bladder tissues from 10 health people were used as control group.[Results]]The positive rate of the expression of livin in BTCC was 76.4%（42/55）,while none of control group expressed livin.The positive rate of the expression of caspase-3 in BTCC was 32.7%（18/55）.The expression of livin was related to the recurrence,but had no significant difference in pathological stages,histological grades,number and metastasis.Caspase-3 had no significant difference in recurrence,pathological stages,histological grades,number and metastasis.There was a negative correlation between the expression of livin and caspase-3 in BTCC.[Conclusion]Positive expression of livin in BTCC is associated with high risk of recurrence,and can be used to monitor early diagnosis and recurrence.High expression of livin and low expression of caspase-3 may play an important role in the occurrence and developement of BTCC.

关 键 词：癌 移行性细胞/病理学 膀胱肿瘤/病理学 脱噬作用 Caspase类/分析 

分 类 号：R737.14[医药卫生—肿瘤]