三氧化二砷协同柔红霉素对K562/A02细胞株的作用  

Synergistic effects of arsenic trioxide in combination with daunorubicin on K562/A02

作　　者：王珂[1] 丁家华[2] 吴玮玮[2] 陈宝安[2] 顾炎[2] 袁鹏[2] 

机构地区：[1]东南大学附属江阴医院检验科,江苏江阴214400 [2]东南大学附属中大医院血液科,江苏南京210009

出　　处：《江苏大学学报（医学版）》2008年第6期487-490,共4页Journal of Jiangsu University：Medicine Edition

摘　　要：目的:研究三氧化二砷(As2O3)及柔红霉素(DNR)联合应用后对人白血病耐药细胞株K562/A02的作用,探讨其应用于临床的可能性。方法:采用MTT比色法测定单用DNR及DNR与不同浓度As2O3合用时的生长抑制效果,用流式细胞术检测胞内DNR浓度及凋亡。结果:DNR对K562/A02细胞的半数抑制浓度(IC50)为15.4 mg/L,加用0.25,0.5,1.0μmol/L As2O3时的IC50分别为1.73,0.78,1.42 mg/L。1 mg/L DNR作用于K562/A02 48 h后的凋亡率为(17.4±2.19)%,0.5μmol/L As2O3作用于K562/A02 48 h后的凋亡率为(53.8±5.6)%,而两药合用后凋亡率为(65.5±6.2)%,但合用后胞内DNR浓度并未增加。结论:低浓度的As2O3与DNR联合应用对K562/A02细胞的抑制为协同作用,其效应与增加药物的诱导凋亡作用有关,而非通过增加胞内DNR浓度。Objective： To explore the cytotoxity of combination of arsenic trioxide and daunorubicin on multidrug resistance of cell line K562/A02, and study the feasibility of combination of two drugs in clinical drug therapy. Methods： MTT assay were used to measure the growth inhibitory effect of DNR with or without various concentration of As2O3. The intracellular concentration of DNR and apoptotic changes were observed by flow cytometry. Results ： The IC50 of DNR on K562/A02 cells was 15.4 mg/L. The IC50 of DNR combined with 0.25,0.5,1.0 μmoL/L As203 on K562/A02 cells were 1.73,0.78,1.42 mg/L respectively. The apoptosis percentage of treating K562/A02 cells with DNR 1 mg/L,As2O3 0.5μmol/L and both for 48 hours were （ 17.4 ±2.19） %, （53.8±5.6） % and （65.5±6.2 ） %. But the intracellular concentration of DNR didn＇t raise after combination of two drugs. Conclusion： As2O3 can present a synergistic effect with DNR on K562/A02 cells. The main mechanism may attribute to induction of apoptosis but not increasing the intraeellular DNR content.

关 键 词：药物疗法 联合 多药耐药 三氧化二砷 柔红霉素 

分 类 号：R733.7[医药卫生—肿瘤]