湖北省HIV-1 B′亚型核心蛋白基因序列分析  

Sequence variation in gag P17/24 region of HIV type 1 predominant subtype B strains circulating in Hubei province

作　　者：陈慧萍[1] 褚小刚[1] 詹发先[1] 汤恒[1] 彭国平[1] 戴涌[1] 彭庭海[1] 龚作炯[2] 

机构地区：[1]湖北省疾病预防控制中心性病艾滋病防治中心,武汉430079 [2]武汉大学人民医院感染科病毒学国家重点实验室

出　　处：《中国公共卫生》2008年第12期1419-1420,共2页Chinese Journal of Public Health

基　　金：湖北省卫生厅科研基金重点项目(JX2A17;JX3A23)

摘　　要：目的分析湖北省人类免疫缺陷病毒(HIV-1)主要流行株核心蛋白(gag)基因P17/24区序列特征,了解其流行特点和变异规律。方法对湖北省HIV-1主要流行区域进行流行病学调查,应用巢式聚合酶链反应对102例HI V-1感染者的gag基因P17/24区进行扩增,对阳性扩增样本进行基因测序和序列分析。结果湖北省共发现4种HI V亚型和重组亚型,B′亚型占82.69%,B′/C重组毒株、CRF01-AE重组毒株各占7.69%,C亚型占1.92%;基因序列分析显示,湖北省HIV-1B′亚型与我国河南和云南省等地的毒株有较高的同源性;氨基酸序列变异分析显示,HIV-1B′亚型毒株gag基因P17/24区发生不同程度的变异,P17突变位点较多,P24较为保守。结论B′亚型依然是湖北省HI V优势流行株,HIV-1在湖北省有加快流行和进一步复杂化的趋势。Objective To study the genetic characteristic and amino acid mutations of HIV-1 and to identify the subtypes and infection sources and the trends of infection of human immunodeficiency virus 1 （HIV-1） predominant circulating strains in Hubei province. Methods Epidemiologic survey was done among HIV-1 carriers found in Hubei area. HIV-1 gag P17/24 region were amplified by nested- polymerase chain reaction（nPCR）. The sequences were determined, and then phylogenetic analysis was performed. Results Four HIV-1 strains or circulating recombinant forms（CRFs） were identified in Hubei province and the subtype B＇ was the predominant. B＇ strains were closely related to B. CN. RL42 from Yunnan and B. CN. 02.02HN from Henan. Amino acid sequence analysis of section gag showed P24 was more conservation than P17. Conclusion The subtype B＇ was still the main epidemic subtypes in Hubei province and the spread of the disease in Hubei province might be speeding up and becoming more complex.

关 键 词：人类免疫缺陷病毒(HIV-1) 亚型 GAG基因 基因变异 

分 类 号：R373.9[医药卫生—病原生物学]