腹泻型肠易激综合征患者外周血T淋巴细胞亚群及血清微量元素含量变化研究  

T lymphocyte subgroups and serum levels of trace elements in patients with diarrhea type of irritable bower syndrome

作　　者：金杭斌[1,2] 顾竹影[1] 赵虹雯[1] 王群燕[1] 吴伟[1] 厉有名[1] 

机构地区：[1]浙江大学医学院附属第一医院,浙江杭州310003 [2]杭州市第一人民医院消化内科,浙江杭州310006

出　　处：《浙江大学学报（医学版）》2008年第6期634-637,共4页Journal of Zhejiang University(Medical Sciences)

摘　　要：目的:通过测定腹泻型肠易激综合征(D-IBS)患者外周血T淋巴细胞亚群及血清Zn、Se、Fe、Cu含量变化,旨在探讨IBS的可能病因及发病新机制。方法:随机抽取30例D-IBS患者和30例健康志愿者的外周血标本,采用流式细胞技术,分别测定CD3、CD4、CD8T淋巴细胞亚群百分比,并计算CD4/CD8比值;采用原子吸收光谱法,测定血清Zn、Fe、Cu水平,用原子荧光分析法,测定血清Se水平。结果:D-IBS组外周血CD4T淋巴细胞水平及CD4/CD8比值较正常对照组明显降低(P<0.01);D-IBS组与正常组血清Zn、Se、Fe、Cu水平差异无统计学意义(P>0.05)。结论:外周血T淋巴细胞CD4水平及CD4/CD8比值降低提示D-IBS患者可能存在细胞免疫异常;D-IBS患者血清Zn、Se、Fe、Cu水平较正常对照组无统计学意义。Objective： To investigate the T lymphocyte subgroups and the levels of serum zinc （Zn）, selenium （Se）, iron （Fe）, copper （Cu） in patients with diarrhea type of irritable bower syndrome （D-IBS）. Methods： A total of 30 D-IBS patients and 30 control subjects were enrolled in this study, and their peripheral blood samples were collected. The percentage of peripheral CD3,CD4,CD8 T lymphocytes were analyzed by flow cytometry,and the ratio of CD4/CD8 was calculated. Serum Zn, Fe and Cu levels were determined by atomic absorption spectrometry （AAS）, and the Se level by atomic fluorometry. Results： Compared with control group, the percentage of CD4 T lymphocyte and the ratio of CD4/CD8 in D-IBS group were significantly lower （P〈0. 01）.However,there was no significant difference in serum Zn,Se,Fe,Cu levels between two groups （P 〉 0. 05）. Conclusion： The declines of peripheral blood CD4 T lymphocytes and the ratio of CD4/CD8 may suggest a cellular immune abnormity in D-IBS patients. There was no significant difference in trace elements levels between the two groups.

关 键 词：肠易激综合征/免疫学 T淋巴细胞亚群/免疫学 腹泻/病因学 腹泻型肠易激综合征 微量元素 

分 类 号：R574.4[医药卫生—消化系统]