多聚赖氨酸与EDTA的交联物防治兔眼后囊膜混浊的研究  

The prevention of polylysine-EDTA conjugate for posterior capsule opacification of rabbit

作　　者：杨金玲 张海燕 张苏炯 夏园玲 于燕妮 

机构地区：[1]贵阳医科大学附属医院眼科,550004

出　　处：《眼科研究》2008年第12期893-896,共4页Chinese Ophthalmic Research

基　　金：贵州省科技厅攻关项目资助(黔科合JY字[2005]3007)

摘　　要：目的观察在白内障术中应用多聚赖氨酸与EDTA的交联物对晶状体后囊膜混浊(PCO)的影响。方法利用EDC将EDTA与多聚赖氨酸结合,制成交联物PLE。日本大耳白兔9只(18眼),随机分为3组。实验组在白内障囊外摘出术中分别于囊袋内注入药物:EDTA组注入EDTA20mmol/L;PLE组注入PLE其中含EDTA10mmol/L、NH2-10mmol/L;对照组囊袋内不注入药物。术后用裂隙灯观察眼内组织的变化。28d后处死兔子,摘除眼球,制作病理切片,光镜下观察晶状体上皮细胞(LECs)增生情况。结果PLE组仅有少量细胞残留,未见细胞增生,其他组均可见不同程度的细胞残留及增生。结论与EDTA相比,PLE可更有效地清除LECs。Objective Posterior capsular opaeifieation （PCO） surgery. The major cause of PCO is the proliferation of residual lens is one of the most common complications after cataract epithelial cells （LECs） after surgery. This study was to determine the feasibility of using a polylysine-EDTA （PLE） conjugate to lens capsule during surgery to remove LECs and its prevention for PCO. Methods EDTA was conjugated to polylysine using （1-ethyl-3-[ 3-dimethylaminopropyl ] carbodiimide hydrochloride following the instructions of Pierce. Extracapsular cataract extraction （ECCE） was performed on 18 eyes of 9 Japanese white rabbits,and 5 mL of 20 mmol/L EDTA or PLE containing 10 mmol/L EDTA was injected into the capsular bag separately in 6 eyes for 2 minutes and removed using compound sodium chloride injection, and the equal amount compound sodium chloride injection was used in 6 control eyes. Ocular anterior segment inflammation,including cornea, anterior chamber and posterior capsule in postoperation was assessed under the slitlamp microscope. The rabbits were sacrificed and the eyes were enucleated in the 28th day and the section of eye tissue was prepared for histological examination under the light microscope. Results Some inflammation was seen in the all 18 eyes in 3 days following surgery and diminished 2 weeks later following the use of antimicrobial. Obvious cellular proliferation was seen in the anterior capsular membrane, showing the spindle line of cells over 10 layers at equator of lens in the control group. A severer cellular proliferation was seen in the EDTA group. However,Only a few residual cells were found and there was not obvious proliferation on the anterior capsular membrane in PLE group. Conclusion PLE presents an ideal result in removing the residual LECs and decreasing the proliferation in comparison with EDTA.

关 键 词：多聚赖氨酸 EDTA 后囊膜混浊 晶状体上皮细胞 白内障囊外摘出术 

分 类 号：R779.66[医药卫生—眼科]