银杏叶提取物诱导大鼠主动脉平滑肌细胞HO-1的表达及细胞信号通路研究  

Correlative cell signaling pathway for expression of heme oxygenase-1 induced by ginkgo biloba extract in rat vascular smooth muscle cells

作　　者：杨晓瑜[1] 汪红霞[1] 魏宗德[1] 黄维义[1] 

机构地区：[1]泸州医学院附属医院心内科,四川泸州646000

出　　处：《中国病理生理杂志》2008年第12期2324-2327,共4页Chinese Journal of Pathophysiology

基　　金：四川省自然科学基金资助项目(No.04jy029-021-3)

摘　　要：目的:旨在研究银杏叶提取物(Ginkgo Biloba Extract,EGB761)对大鼠主动脉平滑肌细胞(RVSMC)血红素氧合酶-1(HO-1)蛋白的影响,并探讨其中涉及的细胞信号通路。方法:大鼠主动脉平滑肌细胞株复苏、传代培养到第6代,再复孔培养用于实验,分别给予空白对照、单纯EGB761、EGB761+锌原卟啉Ⅸ(Zn-PPⅨ)或不同的细胞内信号途径特异性阻断剂进行处理,采用Western blotting法定量检测HO-1蛋白表达。结果:EGB761能呈剂量依赖性诱导HO-1蛋白表达,加用ZnPPⅨ(血红素氧合酶特异性阻断剂)及酪氨酸蛋白激酶(TPK)阻断剂木黄酮均能显著抑制EGB761诱导的HO-1蛋白表达(均P<0.01),但calphostin-C(蛋白激酶C阻断剂)、LY294002(磷脂酰肌醇-3激酶阻断剂)及Bay11-7082(核因子-κB阻断剂)对EGB761诱导的HO-1蛋白表达无明显影响(均P>0.05)。结论:(1)EGB761能显著诱导RVSMC中HO-1蛋白的表达,并且这种诱导作用能被血红素氧合酶的特异性阻断剂ZnPPⅨ所阻断。(2)EGB761通过TPK途径介导大鼠主动脉平滑肌细胞HO-1蛋白的表达。AIM ： To explore the effects of heme oxygenase - 1 （ HO - 1 ） protein expression induced by ginkgo biloba extract （ EGB761 ） in rat vascular smooth muscle cells （ RVSMC ） and the correlative cell signaling pathway. METHODS： The RVSMC lines were revived. Serial passage to 6 generation was carried out and divided into different groups. The cells were treated respectively with vehicle, purely EGB761, EGB761 plus zinc protoporphyrin Ⅸ or other specific inhibitors of cell signaling pathway. Western blotting method was used to detect the expression of HO - 1 in RVSMC. RESULTS： EGB761 induced HO- 1 protein expression in a dose dependent manner. ZnPPIX and genitein significantly inhibited HO - 1 protein expression induced by EGB761 （0. 10 ±0.01, 0. 07 ±0. 01 vs 0. 61 ±0. 07, P 〈0. 01, respectively）. However, calphostin -C, LY294002, Bayl 1 - 7082 had no apparent effects on HO - 1 protein expression induced by EGB761 （0.63±0.07, 0.65±0.07, 0.64±0.06 vs 0.61±0.07, P 〉0.05, respectively）. CONCLUSION： （1） EGB761 significantly induces HO -1 protein expression in RVSMC, and the effect can be inhibited by a specific HO inhibitor ZnPPIX. （2） The HO - 1 protein expression induced by EGB761 in RVSMC is mediated by tyrosine protein kinase pathway.

关 键 词：二裂银杏 血红素氧合酶-1 血管平滑肌细胞 信号转导 

分 类 号：R363[医药卫生—病理学]