人参二醇组皂苷对抗二次打击诱导大鼠急性肺损伤  

Panaxadiol saponins alleviates acute lung injury in the two-hit rat model with hemorrhagic shock and lipopolysaccharide

作　　者：于振香[1] 刘喜春[2] 赵雪俭[2] 

机构地区：[1]吉林大学第一医院呼吸内科,吉林长春130021 [2]吉林大学基础医学院病理生理教研室,吉林长春130021

出　　处：《中国病理生理杂志》2008年第12期2402-2406,共5页Chinese Journal of Pathophysiology

基　　金：吉林省科委自然科学基金资助项目(No.20030551-3)

摘　　要：目的:通过失血性休克复合脂多糖(LPS)二次打击建立稳定的急性肺损伤模型并研究人参二醇组皂苷(PDS)的抗损伤作用。方法:40只成龄Wistar大鼠随机分为假手术组(S)、二次打击模型组(HL)、地塞米松(Dex)预治疗组(HLD)和人参二醇组皂苷预治疗组(HLP)。观察各组大鼠的平均动脉压(MABP)的变化及肺脏病理学改变,通过血清酶学检测分析各主要脏器功能变化,采用放射免疫分析法(RIA)测定了血清炎症介质TNF-α和IL-6水平。结果:失血性休克-LPS二次打击造成了大鼠MABP明显下降,血清酶学指标AST、ALT、ALP、LDH、CK水平升高,血清TNF-α和IL-6的含量增高;肺组织结构出现破坏,出现明显炎症反应。与HL比较,HLD及HLP二次打击1 h后MABP逐渐回升,血清酶学指标及TNF-α和IL-6含量明显降低,肺间质炎症明显减轻。结论:大鼠失血复合内毒素注射可成功建立稳定的急性肺损伤模型。与地塞米松作用相似,PDS可升高二次打击大鼠MABP,逆转血清酶水平变化并降低血清中TNF-α和IL-6含量,从而对抗失血及LPS导致的肺损伤。AIM： To establish the two- hit rat model with hemorrhage and lipopolysaccharide （LPS） and to study the effect of panaxadiol saponins （PDS） against acute lung injury. METHODS： Forty Wistar rats were divided randomly into 4 groups： sham operational group（S） ; two - hit groups with hemorrhagic shock - LPS （HL） ; dexamethasone pretreatment group （HLD） and PDS pretreatment group （HLP）. The mean arterial blood pressure （MABP） was monitored dynamically by 4 - channel physiological meter RM - 6000, and pathological alteration of lung tissues was also observed. The leveLs of various serum enzymes, TNF-α and IL - 6 were detected. RESULTS ： MABP decreased in two - hit rat model with hemorrhage - LPS. The serum levels of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, lactate dehydrogenase, creatine kinase, TNF-α and IL- 6 increased significantly. Severe inflammatory change of pulmonary interstitium in HL group was also observed. CONCLUSION： Endotoxin injection following hemorrhage can be used to establish the animal model of acute lung injury. Similar with dexamethasone, PDS prevents lung tissue from serious- ly damage through increasing MABP and decreasing the level of serum enzymes, TNF-α and IL - 6 levels.

关 键 词：急性肺损伤 二次打击 脂多糖类 人参属 地塞米松 

分 类 号：R363[医药卫生—病理学] R605.971[医药卫生—基础医学]