肺组织β-AR和GRK2与重症急性胰腺炎肺损伤的关系以及甲强龙的影响  

Relationships of β-AR and GRK2 in lung,lung injury in rats with severe acute pancreatitis and therapeutic effect by methlyprednisolone

作　　者：叶振宇[1] 李军成[1] 田斌[1] 龚巍[1] 

机构地区：[1]苏州大学附属第二医院普外科,江苏苏州215004

出　　处：《中国病理生理杂志》2008年第12期2412-2417,共6页Chinese Journal of Pathophysiology

摘　　要：目的:探讨重症急性胰腺炎(SAP)致急性肺损伤(ALI)大鼠肺组织β-AR和G蛋白偶联受体激酶2(GRK2)的变化规律以及甲强龙的影响。方法:SD大鼠36只,随机分为3组,每组12只。对照组,仅做十二指肠翻动;模型组,胰胆管逆行注射5%牛磺胆酸钠(1 mL/kg),建立SAP模型;干预组,建立SAP模型后1 h给予甲强龙(30 mg/kg)。于6 h和12 h分别处死大鼠6只取肺组织,放射配基结合实验测量肺组织β-AR最大结合容量Bmax和平衡解离常数Kd,免疫荧光法检测肺组织GRK2表达。结果:模型组和干预组胰腺组织损伤评分显著高于对照组,模型制备成功;模型组和干预组肺组织损伤评分显著高于对照组,发生SAP肺损伤;模型组β-AR Bmax显著低于对照组和干预组,Kd显著高于对照组和干预组;模型组GRK2的表达显著高于对照组和干预组。结论:SD大鼠肺组织有丰富的GRK2表达,SAP肺损伤大鼠肺组织GRK2表达显著增加,这可能是β-AR下调的重要机制。AIM ： To investigate the variation of β- AR in pulmonary microvascular endothelial cells and variation of GRK2 in lung and to explore the therapeutic effect of methlyprednisolone in severe acute pancreatitis - associated lung injury model in rats. METHODS： 36 rats were divided into three groups randomly： the control group, the experimental group, and the intervention group. In the experimental group, severe acute pancreatitis -associated lung injury model was induced in SD rats by retrograde injection of 5% sodium taurocholate into biliopancreatic duct. In the control group, laparotomy was performed, duodenum and pancreas were flipped only. In the intervention group, methlyprednisolone （30 mg/kg） was injected into rump muscle of rats after model developed. At 6 and 12 h after model was developed, the maximum binding capacity （ Bmax ） and the Kd value of β- AR were detected in lung by means of radioactive ligand binding assay. GRK2 expression was detected in lung by means of immunofluorescence. RESULTS： The scores of the severity of pancreatitis and the severity of lung injury in the experimental group were obviously higher than those in control group. In the experimental group, Bmax was obviously lower, Kd and GRK2 were obviously higher than those in control group and the intervention group. CONCLUSION ： The β- AR in lung is lower down and GRK2 expression in lung is up - regulated in severe acute pancreatitis - associated lung injury model in rats. The therapeutic effect of methlyprednisolone to severe acute pancreatitis- associated lung injury is positive.

关 键 词：胰腺炎 肺损伤 受体 肾上腺素能β G蛋白偶联受体激酶2 甲泼尼龙 

分 类 号：R576[医药卫生—消化系统]