克罗恩病患者与健康人血清蛋白质组学比较初步研究  

Proteomics analysis of sera from Crohn's disease patients and healthy individuals

作　　者：康亮[1] 黄美近[1] 杨祖立[2] 王磊[1] 刘炜[3] 黎明涛[3] 汪建平[1] 

机构地区：[1]中山大学附属第六医院结直肠外科,广东广州510655 [2]中山大学附属第六医院胃肠外科,广东广州510655 [3]中山大学基础医学院蛋白质组学实验室,广东广州510080

出　　处：《中国病理生理杂志》2008年第12期2433-2438,共6页Chinese Journal of Pathophysiology

基　　金：广东省自然科学基金资助项目(No.06104601);广东省自然科学基金博士启动资助项目(No.06300805)

摘　　要：目的:应用蛋白质组学方法在患者血清中寻找与克罗恩病相关的蛋白质。方法:采取克罗恩病患者以及正常成人血清蛋白样本各4例,用不同的CyDye荧光染料标记后进行胶内差异双向凝胶电泳(2-D DIGE),并对获得的图谱进行分析及对差异蛋白质进行基质辅助激光解吸离子化飞行时间质谱(MALDI-TOF-MS)鉴定和生物学信息分析。结果:通过2-D DIGE分析,发现了克罗恩病患者中存在29个表达异常蛋白质点,质谱分析和数据库检索共鉴定出22种蛋白质,包括SER/THR,CD45,APC等。结论:蛋白质组学能很好显示克罗恩病患者与正常人血清蛋白质表达差异,本研究鉴定的蛋白质可能为研究克罗恩病的生物学行为提供新的分子标记物。AIM： To explore differentially expressed proteins of sera from Crohn＇s disease patients with comparative proteomics technology. METHODS： Two - dimensional differential in - gel electrophoresis was used to define patterns of protein expression in serum cross - labeled with variant CyDye from 4 patients and matched health adults. Proteins that showed differential expressions were analyzed by matrix - assisted laser desorption/ionization time of flight mass spectrometry. RESULTS ： Two - dimensional cross - labeled with variant CyDye protein maps of Crohn＇s disease patients and . the health adults were gained successfully. Gel -analysis software identified differently expressed 29 spots Crohn＇s disease compared with that of health adults, 22 of which were identified by using mass spectrometry, including serine/threonine - protein kinase ATR, leukocyte common antigen precursor CD45, 60 kD SS - A/Ro ribonucleo - protein, etc. CONCLUSION： Proteomic analysis can identify the serum proteins with variance of Crohn＇s disease versus health, the different expressed proteins may providing probable new biomarkers correlated with biological behavior of Crohn＇s disease.

关 键 词：CROHN病 蛋白质组 

分 类 号：R363[医药卫生—病理学]