异相睡眠剥夺对大鼠脑内质网应激相关蛋白ATF4表达的影响  

Effects of rapid eye movement sleep deprivation on expression of activating transcription factor 4 in rat brain

作　　者：江波[1] 赵忠新[1] 

机构地区：[1]第二军医大学长征医院神经内科,上海200003

出　　处：《中华神经医学杂志》2008年第12期1220-1223,共4页Chinese Journal of Neuromedicine

基　　金：上海市科技发展基金（024119029）

摘　　要：目的研究异相睡眠（REM睡眠）剥夺对大鼠海马及额叶内质网应激相关蛋白转录活化因子4（ATF4）表达的影响。方法大鼠采用完全随机数字表法分为6组：空白对照组（CC组）、环境对照组（TC）、睡眠剥夺（SD）6h、12h、1d、3d组，每组10只。采用改良多平台睡眠剥夺法建立REM睡眠剥夺大鼠模型，免疫组织化学及Western blot方法测定不同时间点大鼠额叶及海马ATF4蛋白的表达。结果CC组大鼠海马及额叶均未检测出ATF4。TC组海马、额叶均有ATF4表达，SD组海马、额叶6h时开始表达ATF4，12h后达到高峰，1d、3d呈逐渐下降趋势。结论REM睡眠剥夺可诱发内质网应激，睡眠剥夺后大鼠海马与额叶发生内质网应激的时间及发展趋势基本相同。Objective To investigate the expression of endoplasmic reticulum stress-related protein activating transcription factor 4 （ATF4, also known as CAMP-response element protein 2） in rat brain after rapid eye movement （REM） sleep deprivation. Methods Using a modified multiple platform method, REM sleep deprivation for different time lengths was induced in 4 groups of rats （n=10, for 6, 12, 24, and 72 h, respectively）, with 10 normal rats as the cage control and another 10 as the tank control groups. Immunohistochemistry and Western blotting were performed to detect ATF4 protein expression in the hippocampus and frontal lobe of the rats. Results No ATF4 was detected in the cage control group. ATF4 expression was detected in the brain tissues of the rats in the tank control group, and the expression peaked after REM sleep deprivation for 12 h, followed by gradual declination as the sleep deprivation was prolonged to 24 and 72 h. Conclusion REM sleep deprivation can induce endoplasmic reticulum stress. The timing of endoplasmic reticulum stress onset and the course of its development following sleep deprivation are almost identical in the frontal lobe and hippocampus of rats.

关 键 词：睡眠剥夺 内质网应激 额叶 海马 应激转录活化因子4 

分 类 号：R741[医药卫生—神经病学与精神病学]