骨桥蛋白在宫颈癌中的表达及临床意义  

Expression and Clinical Significance of Osteopontin in Cervical Cancer

作　　者：田向文[1] 

机构地区：[1]山东省东营市胜利油田中心医院妇产科,257055

出　　处：《青岛医药卫生》2008年第6期404-406,共3页Qingdao Medical Journal

摘　　要：目的探讨骨桥蛋白(Osteopontin)在正常宫颈组织、宫颈上皮内瘤变(CIN)及宫颈浸润癌中的表达,了解其与宫颈癌的发生、转移和侵袭的关系。方法采用免疫组织化学链霉素抗生素蛋白-过氧化物酶(SP)染色法,检测Osteopontin在30例正常宫颈组织、80例宫颈上皮内瘤变组织和72例宫颈癌组织的表达。结果Osteopontin在正常宫颈组织、CIN组织和宫颈浸润性鳞癌的阳性表达率分别是10%(3/30)、38.75%(31/80)和59.72%(43/72),有显著性差异,且呈上升趋势(P<0.05)。在组织学分级方面,Os-teopontin在高、中、低分化的阳性表达率分别为38.89%(7/18)、59.46%(22/37)和93.3%(14/15),χ2检验有显著差异(P<0.05),且随着分化程度的降低,Osteopontin的阳性表达率明显上升。在浸润深度方面,Osteopontin在≤1/2肌层和>1/2肌层的阳性表达率分别为44%(22/50)和95.45%(21/22),存在显著差异(P<0.05)。Osteopontin在临床Ⅰ期和≥Ⅱ期的阳性表达率分别为51.7%(30/58)和92.9%(13/14),有显著性差异(P<0.05)。Osteopontin在宫颈癌组织中无淋巴转移和有淋巴转移的阳性表达率分别为54.68%(35/64)和100%(8/8),也存在显著差异,有统计学意义。结论Osteopontin的表达水平与宫颈癌的组织分化、浸润深度、淋巴转移和临床分期有一定的相关性,可能成为今后判断宫颈癌预后有价值的肿瘤标志物。Objective To investigate the expression of Osteopontin （OPN） in the normal cervix of the uterus organization,the cervix of the cervical intraepithelial neoplasia （CIN） and the cervix of the infiltrating cervical cancer and the relationship with the invasion and metastasis in cervical cancer. Methods The expression of Osteopontin was examined by immuohistochemistry S-P method in 30 cases of normal cervical epithelia,80 cases of CIN and 72 cases of infiltrating cervical cancer. Results The positive expression rate in the normal cervical organization,the CIN and the infiltrating cancer organization was 10% （3/ 30）,38.75%（31/80） and 59.72% （43/72）. There was significant difference and a trend of rising（P〈 0. 05）. By pathological grade, the positive expression of Osteopontin in the well,moderate and poor grade was 38. 89% （7/18）,59. 46% （22/37） and 93.3% （14/15）. There was significant difference by X^2examination. In invasion aspect, the positive expression of the cases which was smaller than 1/2 cevical muscle was 44%,while 95. 45% in the cases which was bigger than 1/2 cevical muscle. There was significant difference by X^ examination. The positive expression of the cases of clinic period Ⅰ and Ⅱ , Ⅲ , Ⅳ was 51.7% （30/58）and 92.9% （13/14）. There was significant difference. The positive expression of the cases without and with lymph node metastasis was 54.68% （35/64） and 100% （8/8）. There was significant difference（P〈0.05）. Conclusion The expression of Osteopontin in cervical cancer is associated with the pathological grade,invasion, clinic period and lymph node metastasis in cervical cancer. Osteopontin expression levels might be used as valued tumor prognosis of cervical cancer later.

关 键 词：骨桥蛋白 免疫组织化学 宫颈上皮内瘤变 宫颈浸润癌 

分 类 号：R737.33[医药卫生—肿瘤]