养心通脉片对家兔动脉硬化血管中细胞周期蛋白E及其依赖性激酶2的影响  

Effect of Yangxin tongmai tablets on the expression of cyclin E and cyclin dependent kinase 2 in rabbit atherosclerosis model

作　　者：黄献平[1] 袁肇凯[1] 杨涛[1] 黄正新[1] 

机构地区：[1]湖南中医药大学,湖南长沙410007

出　　处：《心血管康复医学杂志》2008年第6期584-586,共3页Chinese Journal of Cardiovascular Rehabilitation Medicine

基　　金：湖南省自然科学基金(04C0263);湖南省教育厅重点项目(04A039);湖南省中医管理局项目(202003)

摘　　要：目的:观察细胞周期蛋白E(Cyclin E)及其依赖性激酶(CDK)2在家兔动脉粥样硬化(AS)血管组织中的表达及养心通脉片的干预作用。方法:参照"高脂饲养喂饲法"造模,设养心通脉片组和辛伐他汀片组、模型组(高脂饮食喂养)和健康对照组,分别用养心通脉片、辛伐他汀片稀释液及蒸馏水灌胃。30 d后,采用免疫组化法检测四组血管组织中CyclinE和CDK2的表达强弱。结果:养心通脉片组CyclinE和CDK2的表达明显低于模型组(P<0.01或<0.05),而与健康对照组、辛伐他汀片组比较差异无显著性;但辛伐他汀片组CyclinE和CDK2的表达显著高于健康对照组(P<0.05)。结论:动脉粥样硬化与Cyclin E和CDK2表达增加有关,养心通脉片能调节Cyclin E和CDK2的表达,从而可能抑制细胞的异常增殖,有效地逆转或消退动脉粥样硬化斑块。Objective： To investigate the effect of yangxin tongmai tablets （YTT） on the expression of cyclin E and cyclin dependent kinase 2 （CDK2） in the vascular intima of rabbit atherosclerosis models. Methods： New zealand rabbits were divides into four groups： Yangxin tongmai group, simvastatin group, model group and healthy control group, then fed with high-fatty diet except healthy control group. After 30 days, all rabbits were killed and the aorta was dissected, the expression of cyclin E and CDK2 were detected by immunohistochemistry assay. Results： The expression of cyclin E and CDK2 in yangxin tongmai group was lower than those in model group （P〈0.01 or P〈0.05）, but there was no significant difference compared with healthy control group and simvastatin group （P〈0.05）. Furthermore, compared with the healthy control group, the expression of cyclin E and CDK2 was higher in simvastatin group （P〈0.05）. Conclusion： The result indicated that the expression of cyclin E and CDK2 is enhanced in atherosclerosis, yangxin tongmai tablets can downregulate its expression, possibly resuh in the inhibition of vascular smooth muscle cell proliferation and attenuation of the atherosclerotic plaque.

关 键 词：动脉硬化 养心通脉片 细胞周期蛋白E 周期蛋白依赖性激酶2 

分 类 号：R735.2[医药卫生—肿瘤] R259.414[医药卫生—临床医学]