缓冲液提取害虫松毛虫蛹中的总蛋白质  

Protein extraction from pupae of Dendrolimus punctatus walker by buffer solution

作　　者：王晓玲[1] 徐剑辉 

机构地区：[1]中南林业科技大学生命科学与技术学院,长沙410004 [2]湖南省东安县林业局,东安425903

出　　处：《食品科技》2008年第12期107-109,共3页Food Science and Technology

基　　金：国家自然科学基金项目(30070627);湖南省自然科学基金项目(98JJY2010)

摘　　要：采用单因素试验法研究缓冲液法提取害虫松毛虫蛹中总蛋白质的工艺。结果表明,固液比、抽提时间、抽提温度和沉淀pH对该虫蛹蛋白质的提取率有显著影响(P<0.05),而沉淀温度和沉淀时间对提取率无显著影响。单因素试验的最佳工艺为:固液比为1∶20(w/v)、抽提时间为120 min、抽提温度为45℃,沉淀温度为25℃、沉淀pH为4.5、沉淀时间为30 min。采用最佳工艺获得蛋白产品的粗蛋白含量为81.2%,总氮提取率为48.33%。The protein from pupae of Dendrolimus punctatus walker was extracted by buffer solution with single factor test design. The results showed that relative amount of materials and extraction solvent, extraction temperature, extraction time and precipitation pH had significant effects on the extraction rate of the protein （P 〈0.05）, while precipitation temperature and precipitation time had no significant effects on it. The optimal condition was as follows： relative amount of materials and extraction solvent was 1：20（w/v）, extraction time was 120 min, extraction temperature was 45 ℃, precipitation temperature was 25 ℃, precipitation pH was 4.5, precipitation time was 30 min. The protein content of the product obtained at the optimal condition was 81.2%, and the extraction rate was 48.33%.

关 键 词：害虫 马尾松毛虫 蛹 蛋白质 缓冲液 提取 

分 类 号：Q966[生物学—昆虫学] TS201.2[轻工技术与工程—食品科学]