C型乙型肝炎病毒基因SOEing法引入228位点突变  

Research of Mutation for 228 Base Point of HBV Genotype C

作　　者：徐晓雪[1] 房凯[1] 钟连生[1] 潘忠诚[1] 吴土焕[1] 赵雨杰[1] 

机构地区：[1]中国医科大学基础医学院生物芯片中心,沈阳110001

出　　处：《中国医科大学学报》2008年第6期736-738,共3页Journal of China Medical University

基　　金：辽宁省教育厅高校科学研究计划项目(202011001)

摘　　要：目的为获取乙型肝炎病毒(HBV)基因分型芯片病毒靶序列,对C型HBV第228位碱基进行点突变。方法通过不同基因型HBV全基因组的序列比对分析,显示基因型A,C,D,G在228位上的碱基为G,而基因型B,E,F,H为A,所以检测该位点的碱基类型可以实现HBV基因型的初步分型。结果将C型乙肝病毒第228位上的单位碱基由G突变为A,经测序后证明突变成功。结论重叠区扩增基因拼接法(SOEing)是一种简便引入点突变的方法,可以应用此法制备HBV基因分型芯片检测基因片段序列。Objective To get HBV DNA sequences for HBV genotyping microarray by mutation of the 228 base of HBV. Methods By comparing different HBV DNA genome sequences,it was shown that the 228 base point in genotype A, C, D, G was G,while the genotype B,E,F,H was A. Gene splicing by overlap extension （SOEing）method was used to mutate the 228 base of HBV genotype C by gene,and the sequences were confirmed by sequencing. Results The 228 base of HBV genotype C was successfully mutated from G to A. Conclusion SOEing method is a convenient and easy way to make mutation, and this method can be used to prepare HBV target sequences which can be used for HBV genotyping by microarray analysis.

关 键 词：乙型肝炎病毒 基因分型 重叠区扩增基因拼接法 

分 类 号：R512.6[医药卫生—内科学]