MMP-9在ApoE基因敲除小鼠主动脉粥样硬化斑块的表达及高脂饮食影响  

The Expression of MMP-9 and the Effect of Hight Cholesterol Diet in ApoE-Deficient Mice

作　　者：朱杰[1] 汪茜[2] 张旭[2] 周君[3] 

机构地区：[1]中国医科大学附属第四医院急诊科,沈阳110032 [2]中国医科大学第90期临床医学系,沈阳110001 [3]美国加州大学耳湾医学中心心血管实验室

出　　处：《中国医科大学学报》2008年第6期750-752,共3页Journal of China Medical University

摘　　要：目的研究基质金属蛋白酶9(MMP-9)在载脂蛋白E(apolipoprotein E,ApoE)基因敲除C57BL/6J小鼠[ApoE(-/-)]主动脉粥样硬化斑块中的表达及高脂饮食对其影响,分析其与动脉粥样硬化不稳定斑块的关系。方法16只30周龄ApE(-/-)小鼠,随机分成高脂饮食组[Ch-ApoE(-/-)]和普通饮食组[ApoE(-/-)],8只正常C57BL/6J小鼠为对照组予高脂饮食,喂养4个月后分离主动脉。HE、Masson三色法染色观察小鼠主动脉粥样斑块,免疫组织化学分析斑块内MMP-9及巨噬细胞的表达。结果两组ApoE(-/-)均有明显的动脉粥样硬化,为不稳定斑块,斑块内巨噬细胞表达过量,且MMP-9表达增强。Ch-ApoE(-/-)组动脉硬化及MMP-9表达强于ApoE(-/-)组,两者有统计学意义。结论动脉粥样硬化不稳定斑块与MMP-9的表达密切相关,高脂饮食可加重不稳定斑块。Objective To study the expression of matrix metalloprotein-9 （MMP-9） in atherosclerosis plaques of apolipoprotein E-deficient mice and the impact of high cholesterol diet on it and to investigate whether high cholesterol diet is involved in unstable plaques. Me,otis 16 ApoE-deficient mice at 30 weeks of age were divided randomly into high cholesterol diet group（n=8） and normal diet group （n=8）, with 8 normal wild-type C57BL/6J mice as a control group. After the mice were fed for 4 months, their arterial aortas were separated, and the atherosclerosis lesions tissues were observed with HE staining and Masson staining. The expressions of MMP-9 and macrophage were analyzed immunohistochemically. Results Atherosclerosis lesions and unstable plaques were seen clearly in the Ch-ApoE（-/-）and ApoE（-/-）. The expressions of macrophage and MMP-9 in the unstable plaques of Ch-ApoE（-/-）were significantly higher than that of ApoE（-/-）. Conclusion The expression of MMP-9 is closely related to the unstable atherosclerosis plaques. High cholesterol diet can aggravate the damage.

关 键 词：动脉粥样硬化 基质金属蛋白酶9 载脂蛋白E基因敲除小鼠 高脂饮食 

分 类 号：R543[医药卫生—心血管疾病]