不同类型胃黏膜肠上皮化生CDX2蛋白的表达及幽门螺杆菌感染对其表达的影响  

Expression of CDX2 and its Relationship with Helicobacter Pylori in Different Types of Intestinal Metaplasia of Gastric Mucosa

作　　者：王旭光[1,2] 张忠[2] 张晔[1] 袁媛[1] 

机构地区：[1]中国医科大学附属第一医院普外科暨肿瘤研究所肿瘤病因与预防实验室,沈阳110001 [2]沈阳医学院病理学教研室,沈阳110034

出　　处：《中国医科大学学报》2008年第6期755-757,共3页Journal of China Medical University

基　　金：国家自然科学基金资助项目(30572131)

摘　　要：目的检测不同类型肠化生CDX2蛋白的表达及幽门螺杆菌(H.pylori)感染对其表达的影响,探讨其在肠化生发生发展过程中的意义。方法利用免疫组化技术检测58例正常胃黏膜、184例肠化生和36例胃癌组织CDX2蛋白表达;利用HID-ABpH2.5-PAS黏蛋白组织化学染色将184例肠化生分为I型81例,Ⅱ型62例和Ⅲ型41例;利用HE染色和ELISA法检测血清H.pylori Ig G,将184例肠化生分为H.pylori阳性组79例,H.pylori阴性105例。结果正常胃黏膜CDX2阴性表达,胃癌组织CDX2表达高于正常胃黏膜(P<0.01),低于肠化生黏膜(P<0.01);Ⅰ型、Ⅱ型肠化生CDX2表达高于胃癌和Ⅲ型肠化生,差异均有统计学意义。H.pylori阳性组CDX2表达高于H.pylori阴性组(P>0.05)。结论CDX2可作为判定肠化生分化程度和判定病变胃黏膜上皮恶变的指标。Objective To investigate the role of CDX2 during the development of IM by detecting its expression in different IM and the expression change in H. pylori infection, and to discuss the significance of CDX2 in different IM. Methods The expression of CDX2 was detected immunohistochemically in 58 cases of normal gastric mucosa, 184 cases of IM and 36 cases of gastric cancer. The IM was divided into three subtypes by HID-ABpH2.5-PAS methods： 81 cases of IM 1,62 cases of IM Ⅱ and 41 cases of IM Ⅲ. The H. priori Ig G was detected by ELISA method and H.E. staining in 184 eases of IM,whieh were divided into 79 cases（H, pylori-positive group） and 105 cases （H. pylori-negative group）. Results The positive rates of CDX2 expression in IM and gastric cancer were significantly higher than that of normal gastric mucosa,whieh was all negative（P 〈 0.01 ）.The positive rate of CDX2 expression of gastric cancer was lower than that of IM（P 〈 0.01 ）. The positive rates of CDX2 expression in IM Ⅰ,IM Ⅱ and IM Ⅲ were decreased,with significant difference between IM Ⅰ and IM Ⅲ （P〈 0.01）,and there was a difference between IM Ⅱ and IM Ⅲ （P〈 0.01）. The positive rate of CDX2 expression in IM Ⅰ and IM Ⅱ had significantly difference compared with gastric cancer group （P 〈 0.01 ,P〈0.05,respectively）,while there was no difference between IM Ⅲ and gaslxic cancer group. CDX2 was located in IM cellular nucleus. The positive rates of CDX2 in H. pylori-positve groups IM were higher than those of H. pylori-negative groups（P 〉 0.05 ）. Conclusion CDX2 is a good index for judging the differentiation degree of IM, and also for judging the gastric mucosa lesion malignancy.

关 键 词：肠化生 CDX2蛋白 胃癌 幽门螺杆菌 

分 类 号：R735.2[医药卫生—肿瘤]