上皮钙黏蛋白和γ-连环蛋白在大肠癌的表达及临床意义  

The Expression and the Significance of E-cadherin and γ-catenin in Colorectal Carcinoma

作　　者：胡长梅[1] 霍继荣[1] 周世权[1] 任剑珍[1] 

机构地区：[1]中南大学湘雅第二医院消化科,长沙410011

出　　处：《中国医科大学学报》2008年第6期773-775,共3页Journal of China Medical University

基　　金：湖南省自然科学基金资助项目(04JJ3107)

摘　　要：目的检测大肠癌组织中上皮钙黏蛋白(E-cad),γ-连环蛋白(γ-cat)的蛋白表达,了解与大肠癌临床病理的相关因素。方法采用免疫组化二步法,检测E-cad及γ-cat在各组织中的表达及意义,并观察临床病理参数。结果E-cad表达阳性率:正常大肠组>大肠腺瘤组>大肠癌组,γ-cat表达阳性率:正常大肠组﹥大肠腺瘤组,正常大肠组﹥大肠癌组;E-cad的膜表达缺失率在大肠癌组织标本中与肿瘤的分化程度、浸润深度、有无淋巴结转移、有无远处转移、Dukes分期有密切关系;γ-cat的蛋白表达缺失率在大肠癌组织标本中与有无淋巴结转移、有无远处转移有关;在大肠癌组织中E-cad及γ-cat的表达率降低,但两者之间相关性分析结果显示无统计学意义。结论大肠癌组织中E-cad和γ-cat的表达下降与肿瘤相关。E-cad的表达与肿瘤的分化、浸润转移有密切的关系,γ-cat的表达与肿瘤转移有关。Objective To determine the expression of E-cadherin/γ-catenin complex in colorectal carcinoma and investigate the relationship between E-cadherin/γ-catenin complex and the clinicopathologic features. Methods 60 cases of tissues were examined ,and the clinical pathologic parameters was detected dicho-step method was used to detect the E-cadherin/γ-catenin and to analyze their significance and the correlation between them. Results The expression rates of E-cadherin： normal colorectal tissues〉 colorectal adenoma tissues 〉 colorectal carcinoma tissues. The expression rates of γ-cateniu： normal coloreetal tissues〉 colorectal adenoma tissues and normal colorectal tissues 〉 colorectal carcinoma tissues. The expression level of E-cadherin was closely related with Dukes stage, metastasis （including lymphatic metastasis and distant place metastasis）, depth of ildiltration and differentiation. The expression level of γ-catenin was closely related with lymphatic metastasis and distant metastasis. The expression of the E-cadherin and γ-catenin both decreased in colorectal carcinoma tissues, but the analysis of their correlation showed there was no statistic significance. Conclusion The expression level of E-cadherin is related to Dukes stage, metastasis （including lymphatic metastasis and distant metastasis ）, depth of infiltration, differentiation. And the expression of γ-catenin is related to lymphatic metastasis and distant metastasis. Detecting their expression might be useful prognostic indicators and give a theoretical help in the therapy of colorectal carcinoma.

关 键 词：大肠癌 上皮钙黏蛋白 Γ-连环蛋白 免疫组织化学 

分 类 号：R574.62[医药卫生—消化系统]