毛脉酸模中二苯乙烯类成分对HepG2细胞肿瘤相关基因表达的影响  

Effects of chrysophenine from Rumex gmelini Turcz on tumor-associated gene expression in HepG2 cells

作　　者：王振月[1] 唐先明[1,2] 赵海鹏[1,3] 王宗权[1,4] 陈智岩[1] 

机构地区：[1]黑龙江中医药大学药学院,黑龙江哈尔滨150040 [2]哈尔滨市食品药品检验检测中心,黑龙江哈尔滨150525 [3]鲁南制药集团股份有限公司,山东临沂276005 [4]以领医药集团医药研究院,河北石家庄050035

出　　处：《第四军医大学学报》2008年第23期2141-2143,共3页Journal of the Fourth Military Medical University

基　　金：黑龙江省自然科学基金重点项目(ZJY005006-02);黑龙江省研究生创新科研基金项目

摘　　要：目的:探讨毛脉酸模提取物二苯乙烯类成分与肝癌发生、发展的关系及其作用机制.方法:用二苯乙烯类成分处理人肝癌细胞株HepG248h后,提取药物处理组和对照组细胞的总RNA,将两组RNA纯化为mRNA,逆转录成cDNA,用Cy3和Cy5两种不同的荧光染料进行线性扩增标记,然后与人肿瘤相关基因表达谱芯片杂交,扫描后对获得的数据用LuxScan3.0软件分析.结果:经二苯乙烯类成分诱导后HepG2细胞的2474个肿瘤相关基因中,有GADD153,HPRG等5个基因表达明显上调,PRL-1,PAI-1等9个基因明显下调.结论:二苯乙烯类成分能改变细胞周期进程、细胞增殖、细胞凋亡、血管生成等相关基因的表达,此可能是其抗肿瘤作用的机制.AIM ：To investigate the effects of chrysophenine fi＇om Rumex gmelini Turez on the cancer-related gene expressions of HepG2 cells by gene expresion profile chip and to study its relationship with the development of hepatic cancer and the relative mechanisms. METHODS： The total RNAs were extracted from chrysophenine untreated or treated HepG2 cells to synthesize doub- le-stranded cDNAs by reverse transcription. Then cDNA labeled with the incorporation of Cy3 and CyS-dUT were amplified linearly. Probes were hybridized with oligonucleotide microarray. The chips were scanned and then analyzed by LuxScan 3. 0 software. RESULTS：After chrysophenine induction, expressions of 9 out of 2474 cancer related genes, including PRL-1, PAI-1, ere, were dowrl-regulated notably. Meanwhile, expressions of 5 out of 2474- cancer genes, including GADD153,HPRG, etc, were up-regulated markedly. CONCLUSION： The alterations in specific genes involved in modulating the cell cycle progress, cell proliferation, cell apoptosis,and antiangiogenesis may be responsible for anticancer molecular mechanism of chrysophenine.

关 键 词：基因表达 毛脉酸模 HEPG2细胞株 二苯乙烯类 

分 类 号：R735.7[医药卫生—肿瘤]