人胰腺癌细胞系表达干细胞标志物研究  

Human pancreatic carcinoma cell lines express markers of stem cell

作　　者：杜志勇[1] 魏翠凤[2] 胡君[1] 王敏[1] 田锐[1] 秦仁义[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院胆胰外科,武汉430030 [2]湖北省荆门市第一人民医院内分泌科

出　　处：《中华实验外科杂志》2008年第12期1606-1608,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（30471693）

摘　　要：目的观察胚胎干细胞和胰腺相关干细胞标志物在胰腺癌细胞系中基因和蛋白水平的表达，探讨胰腺癌起源。方法应用逆转录聚合酶链反应（RT-PCR）检测胚胎干细胞标志物Oct4、abcg2、c-kit以及c-kit配体干细胞因子（SCF）和胰腺相关干细胞标志物角蛋白19（ck19）、巢蛋白（nestin）、波形蛋白（vimentin）、胰十二指肠同源盒基因-1（PDX-1）等在胰腺癌细胞系sw1990、BxPC3、pc3和jf305中基因水平（mRNA）的表达；利用免疫细胞化学（ICC）方法检测Oct4、abcg2、c-kit和ck19、nestin在上述细胞系中蛋白水平的表达。同时结合异硫氰荧光素（FITC）标记抗体进行流式分选检测上述指标在胰腺癌细胞中的表达率。结果除BxPC3、pc3和jf305不表达c-kit以外，上述4种胰腺癌细胞系在mRNA水平表达其余所有的标志物；在蛋白水平，上述4种细胞均表达Oct4、abcg2、ck19、nestin，而除sw1990以外的3株胰腺癌细胞株不表达c-kit。结论人胰腺癌细胞系表达胚胎干细胞标志物Oct4、abcg2和胰腺干细胞标志物ck19、nestin、vimentin、PDX-1，部分表达c-kit。胰腺癌可能来源于成体胰腺干细胞或胰腺前体细胞。Objective To investigate the expression of markers of human pancreas stem cells in four pancreatic cancer cell lines and study the origination of pancreatic cancer. Methods RT-PCR was used to detect mRNA expression of Oct4, c-kit, scf, ckl 9, nestin, vimentin, abeg2, PDX-1, and immunocyto-chemistry was used to detect protein expression of Oct4, c-kit, ck19, nestin, abcg2 in four pancreatic cancer cell lines including sw1990, BxPC3, pc3 and if305. FITC-marked flow eytomytry was used to detect the expression rate of them in four pancreatic cancer lines. Results Four pancreatic cancer cell lines expressed mRNA of all Oct4,scf,ck19,nestin,vimentin, abcg2 and PDX-1. sw1990 expressed mRNA and protein of c-kit,but BxPC3,pc3 and jf305 did not express mRNA and protein of c-kit. Four pancreatic cancer cell lines expressed protein of Oct4, ahcg2, ck19 and nestin. Conclusion Human pancreatic cancer cell lines express markers of human embryonic stem cells including Oct4, abcg2, scf and markers of pancreas stem cells including ck19 ,nestin, vimentin and PDX-1. Some pancreatic cancer cell lines do not expression c- kit. Pancreatic cancer can originate from pancreas stem cells or precursor-cells of pancreas.

关 键 词：胰腺癌 胰腺干细胞 标志物 

分 类 号：R735.9[医药卫生—肿瘤] R364.7[医药卫生—临床医学]