缺氧通过抑制E-cadherin促进胰腺癌细胞侵袭的研究  

Hypoxia promotes the invasive ability of pancreatic cancer cells by suppressiong E-cadherin

作　　者：周峰[1] 殷涛[1] 熊炯圻[1] 吴河水[1] 王春友[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院胰腺外科中心,武汉430022

出　　处：《中华实验外科杂志》2008年第12期1609-1611,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（30571817）

摘　　要：目的观察缺氧对胰腺癌细胞E-cadherin表达的抑制以及侵袭性生物学行为的影响，探讨E-cadherin表达抑制的调控机制。方法分别在常氧和缺氧环境下培养胰腺癌细胞Panc-1，通过Western blot和Transwell技术对比观察E-cadherin表达的差异以及细胞侵袭能力的变化。体外转染表达HIF-α siRNA的真核表达载体pGenesil-1-HIF-1α以及对照载体，进一步检测沉默HIF-α之后，Panc-1细胞E-cadherin和细胞侵袭能力的变化。结果缺氧可以抑制Panc-1细胞E-cadherin的表达，并增强其在体外的侵袭能力。而通过RNAi技术沉默了HIF-α之后，则可以部分恢复缺氧微环境对Panc-1细胞E-cadherin表达的抑制，同时降低其在体外的侵袭能力。结论缺氧微环境中HIF-α的活化可抑制E-cadherin的表达并加强胰腺癌细胞侵袭能力。Objective To investigate the influence of hypoxia on E-cadherin and invasive behaviors of pancreatic carcinoma ceils. Methods The pancreatic cancer cell line Panc-1 was cultured under hypoxia and nomoxia. The expression of E-cadherin was detected by Western blot, and the invasion ability was determined by Transwell assay. The plasmid pGenesil-1-HIF-1α expressing shRNA targeted at HIF-1α gene and the control plasmid were transfected to silence the HIF-1α, and the E-cadherin and the invasive ability were determined accordingly. Results Hypoxia could suppress the expression of E-eadherin and enhance the invasive ability of Pane-1 cells. After silencing the expression of HIF-1α under hypoxia, the expression of E-cadherin could be enhanced and the invasive ability was inhibited. Condusion Hypoxia can promote the invasive ability of pancreatic cancer cells by activating HIF-1α and suppressing E-cadherin. The therapy targeting HIF-1α may provide a new weapon for overcoming pancreatic cancer.

关 键 词：胰腺癌 缺氧 侵袭 

分 类 号：R735.9[医药卫生—肿瘤]