从茶树种胚中提取总RNA方法的研究  

Studies on isolation of total RNA from tea plant embryo samples

作　　者：胥振国[1] 江昌俊[1] 张莉[1] 杨书运[1] 袁支红[1] 谭振[1] 

机构地区：[1]安徽农业大学茶叶生物化学与生物技术重点实验室,合肥230036

出　　处：《生物学杂志》2008年第6期66-68,共3页Journal of Biology

基　　金：安徽省教育厅自然科学研究计划重点项目(2004kj137zd)

摘　　要：茶树种胚中富含多糖和多酚类化合物,这增加了总RNA的提取难度。试验以茶树种胚为研究对象,通过对几种提取总RNA方法的比较与分析,认为用改进的CTAB-LiCl法提取茶树种胚总RNA的产率较高,质量好,可直接用于cDNA的合成、cDNA-AFLP分析等分子生物学实验操作。Tea plant embryos are rich in polysaccharides and polyphenols compounds which increase the difficulties in isolating RNA from them. With tea plant embryos as the research object, we compares and analyses several methods of total RNA extraction. The results show the higher yield and good quality of RNA are extracted using an improved CTAB-LiCl method which can be used directly in cDNA synthesis, cDNA-AFLP analysis and other molecular biological experiment.

关 键 词：苍树种胚 多糖 多酚 总RNA CTAB—LiCl法 

分 类 号：Q503[生物学—生物化学]