SDZ对人大肠癌LOVO细胞系的作用及其对E-、N-Cadherin表达的影响  

Effects of SDZ on LOVO Cell Line and the Expression of E-Cadherin and N-Cadherin

作　　者：吴迪[1] 佟金学[1] 

机构地区：[1]哈尔滨医科大学附属第四医院,哈尔滨市150001

出　　处：《中国药房》2008年第36期2808-2811,共4页China Pharmacy

基　　金：黑龙江省攻关课题(GB05C401-08);黑龙江省普通高等学校骨干教师创新能力资助计划(1055G026)

摘　　要：目的:研究SDZ对人大肠癌LOVO细胞系的作用及其对上皮型、神经型钙黏蛋白(E-、N-Cadherin)表达的影响。方法:用倒置显微镜和电镜观察SDZ对人大肠癌LOVO细胞的凋亡情况,应用逆转录-聚合酶链反应(RT-PCR)检测加药后E-、N-Cadherin的表达情况。结果:SDZ对人大肠癌LOVO细胞株有促进凋亡的作用;对E-Cadherin的表达有上调作用,对N-Cadherin的表达无影响。结论:SDZ有加强肿瘤细胞间的黏附性,降低肿瘤浸润和转移的能力。OBJECTIVE.To investigate the effects of SDZ on LOVO cell line and the expression of E- Cadherin and N- Cadherin. METHODS： The apoptosis of SDZ on growth of LOVO cells was observed under electron microscope and inverted microscope. The expression of E - Cadherin and N - Cadherin treated by SDZ were detected by RT - PCR. RESULTS： SDZ promoted the apoptosis of LOVO cell lines and increased the expression of E-Cadherin, however, it did no effect on the expression of N - Cadherin. CONCLUSION： SDZ can enhance the intercellular adhesiveness and degrade the capability of infil- tration and metastasis of tumor ceils.

关 键 词：SDZ 中药 人大肠癌LOVO细胞株 逆转录-聚合酶链反应 

分 类 号：R730.52[医药卫生—肿瘤] R979.1[医药卫生—临床医学]