微波加热处理在冰冻切片粘液染色中的应用价值  

Value on frozen sections of mucus stainning using microwave heating

作　　者：徐彤[1] 吴秋良[2] 范丽萍[1] 

机构地区：[1]广东省韶关市粤北人民医院病理科,512026 [2]广州中山医科大学肿瘤医院病理科,510060

出　　处：《航空航天医药》2008年第4期203-204,共2页Aerospace Medicine

摘　　要：目的:探讨微波加热处理在冰冻切片粘液染色中的作用。方法:采用微波处理法(MW)与常规法(CT)分别处理60例癌组织标本,并行常规HE和粘液染色(AB)法,显微镜下观察各自冰冻切片的显色效果;记录并比较二者显影时间。结果:经MW处理的冰冻切片染色时间为1min30s(常规均大于15min);前者染色清晰,质量与后者无明显差异。结论:MW较之CT染色制片明显缩短了显影时间,质量稳定,值得推广应用。Objective： To probe the wlue on frozen sections of mucus staining using microwave heating. Methods： The microwave （MW） and conventional treatment（ CT） were dealt with 60 cases of cancer tissue samples. They were stained using conventional HE and mucus staining（AB） .The color effects of their frozen section were observed under the microscope.Their development time were recorded and compared.Results：It spent 1 minutes and 30 seconds to complete frozen section in MW, 〉 15 minutes in CT.Sections of mucus using MW were stained clear.Both quality of MW and CT were no significant difference. Conclusions： MW could more significantly reduce development time than CT. Its quality stable, MW should be applied.

关 键 词：微波 冰冻切片 染色 显影时间 

分 类 号：R365[医药卫生—病理学]