外施H2O2对黄瓜幼苗抗低温胁迫的影响  

Effects of Exogenous H_2O_2 on the Chilling Stress Resistance of Cucumber Seedlings

作　　者：吴顺[1] 赵永进[1] 萧浪涛[2] 罗光宇[1] 

机构地区：[1]中南林业科技大学生命科学与技术学院,湖南长沙410004 [2]湖南农业大学植物激素重点实验室,湖南长沙410128

出　　处：《中南林业科技大学学报》2008年第5期68-70,76,共4页Journal of Central South University of Forestry & Technology

基　　金：教育部青年骨干教师基金项目(2000科教司65号);中南林业科技大学引进人才项目(104-0024)

摘　　要：以"津研7号"和"津春4号"黄瓜幼苗为材料,在其生长期外施不同浓度的H2O2,研究了H2O2对黄瓜幼苗抗低温胁迫的影响.结果表明:黄瓜幼苗经低温胁迫后,其电导率和丙二醛含量急剧上升,而过氧化物酶活性和可溶性蛋白含量明显降低;与对照相比,外施H2O2能提高其过氧化物酶活性,降低膜渗漏率,增加可溶性蛋白含量,减缓膜脂过氧化作用,从而提高黄瓜幼苗的抗低温胁迫能力,其中以10 mmol.L-1的H2O2处理效果最为明显.To study the effects of H2O2 on the chilling stress resistance of cucumber seedlings, this paper made an experiment by spraying different concentrations of H2O2 onto the seedlings and exposing them to 7 ℃ low temperature for 12 h. Results show that under a chilling stress, the seedlings present a sharp increase of electric conductivity and MDA content with an obvious drop of POD activity and soluble protein content ; and that contrasted to the control, exogenous H2O2 increases the POD activities and soluble sugar contents of the seedlings and alleviates their cell membrane leakage and membrane lipid peroxidation during the chilling stress and recovery periods. It is clear that exogenous H2O2 enhances the chilling stress resistance of cucumber seedlings, the effect of 10 mmol · L^-1 H2O2 being most significant.

关 键 词：植物生理学 H2O2 黄瓜幼苗 低温胁迫 

分 类 号：Q945.78[生物学—植物学] S642.2[农业科学—蔬菜学]