多柔比星葡聚糖纳米粒联合YH-16对SKOV3的杀伤作用  

Study on inhibitory effect of doxorubicin encapsulated in amphiphilic polysaccharide nanoparticles combined with YH-16 on Oophoroma

作　　者：李霞[1] 曹翠琴[1] 

机构地区：[1]武汉市第一医院肿瘤科,湖北武汉430022

出　　处：《岭南现代临床外科》2008年第5期390-392,共3页Lingnan Modern Clinics in Surgery

基　　金：武汉市科学技术计划项目(z20036002005)

摘　　要：目的初步探讨载多柔比星葡聚糖纳米粒(DOX/DEX-PLA)联合YH-16(重组内皮抑素)对卵巢癌细胞的体内外的杀伤作用。方法以双乳化剂蒸发法制备DOX/DEX-PLA载药纳米粒,透射电镜观察纳米粒形态,分光光度法计算载药率,体外药物释放实验考察纳米粒对DOX的缓释作用。MTT法观察对卵巢癌细胞的体外杀伤作用,动物实验观察其体内抑瘤效应。结果DOX/DEX-g-PLA纳米微粒呈球形,粒径约83nm,药物包封率约67.1%。体外释放实验提示,纳米制剂中约50%的药物持续缓慢释放,可持续达7d;体内抑瘤实验显示,纳米缓释制剂联合YH-16间隔给药疗效优于未包载药物每日给药的疗效。结论DOX/DEX-PLA纳米粒联合YH-16可有效抑制卵巢癌细胞的生长。Objective To investigate the inhibitory effect of DOX/DEX-PLA nanoparticles combined with YH-16 on oophoroma cells both in vitro and in vivo. Methods The DOX/DEX-PLA nanoparticles were prepared by the method of emulsification ＆ evaporation of organic solvent. Its morphology was observed by transmission electron microscopy and the encapsulating efficiency of DOX was determined by ultraviolet spectrophotometry. The inhibitory effect against oopboroma cells in vitro was measured by MTT assay. Results The DOX /DEX-PLA nanoparticles were found or elliptical shape. The diameter of the nanoparticles was about 83 nm, and the entrapment efficiency was about 67.1%. The releasing test in vitro revealed that over 50 percent of DOX encapsulated in DOX /DEX-PLA nanoparticles were slowly released for about 7 days. The Anti-oophoroma effect in vivo indicated that the efficacy of control-releasing agent combined with YH-16 by interval drug delivery had advantages over one of the unenveloped drug per day. Conlusion DOX /DEX-PLA nanoparticles combined with YH-16 can effectively inhibit the growth of oophoroma cells.

关 键 词：YH-16 葡聚糖 纳米粒子 多柔比星 卵巢癌 抗肿瘤 

分 类 号：R737.31[医药卫生—肿瘤]