真空膜结晶法结晶溶菌酶的实验研究  

Study on vacuum membrane crystallization of lysozyme

作　　者：左俊芳[1] 张新妙[1] 马润宇[1] 

机构地区：[1]北京化工大学生命科学与技术学院,北京100029

出　　处：《膜科学与技术》2008年第6期69-74,共6页Membrane Science and Technology

基　　金：国家自然科学基金资助项目(20676007)

摘　　要：采用聚偏氟乙烯中空纤维微孔膜对真空膜结晶溶菌酶进行了实验研究.考察了膜下游真空度、蛋白质浓度、料液流速、添加剂、pH和离子强度对溶剂跨膜通量及溶菌酶晶体质量的影响.结果表明,真空度应控制在0.015 MPa左右;溶菌酶真空膜结晶的最佳浓度为20 mg/mL;结晶溶液的流速以288μm/s为宜;结晶溶液中加入一定添加剂如丙三醇、DMSO等,可以有效提高溶剂跨膜通量,改善晶体质量;在相同离子强度下,pH值越大膜通量越低,在不同的pH值下可以得到不同形状的晶体.The bench study of lysozyme by vacuum membrane crystallization, using mieroporous hydrophobic PVDF membrane, was carried out. The influences of vacuum tightness of downstream side, protein concentration, flow velocity of feed solution, additive, pH and ionic strength on transmembrane flux and the shape of lysozyme crystal were examined. The results showed that the vacuume should be controlled under 0. 015 MPa; the optimum lysozyme concentration and flow velocity were 20 mg/mL and 288μm/s respectively for vacuum membrane crystallization; the addition of additives such as glycerol and DMSO into the crystallization solutions could increase effectively the transmembrane flux and improve the quality of crystals; Under the same ionic strength, the higher transmembrane flux was obtained under lower pH, different crystal forms could be obtained under different pH.

关 键 词：膜结晶 溶菌酶 跨膜通量 结晶条件 

分 类 号：TQ925[轻工技术与工程—发酵工程]