Characteristic fragmentation behavior of the analogs of endomorphin-2 with phenylglycine in position 3 or 4 by ESI-FT tandem mass spectrometry  

作　　者：Chang Feng Hu Chuan Jun Zhu Yan Feng Gao Xuan Shao Rui Wang Yu Xin Cui 

机构地区：[1]State Key Laboratory of Natural and Biomimetic Drugs （Peking University）, Beijing 100083, China [2]School of Life Science, Lanzhou University, Lanzhou 730000, China

出　　处：《Chinese Chemical Letters》2008年第12期1479-1482,共4页中国化学快报（英文版）

摘　　要：A series of analogs of endomorphin-2 （EM-2） with phenylglycine （Phg） in position 3 or 4 were synthesized. In electrospray ionization Fourier transform ion cyclotron resonance （ESI-Fr-ICR） MS/MS spectra of these compounds, some b, y, a, and internal ions were observed and slight mass differences between the calculated and observed results are obtained. Their sequences were derived successfully. However, the MS/MS patterns of these analogs with Dphg and Lphg were very similar. It is hard to distinguish them by MS/MS spectra. Moreover, if the third position was substituted by phenylglycine （L or D）, a rearrangement could occur in MS/MS experiment to lose proline residue.A series of analogs of endomorphin-2 （EM-2） with phenylglycine （Phg） in position 3 or 4 were synthesized. In electrospray ionization Fourier transform ion cyclotron resonance （ESI-Fr-ICR） MS/MS spectra of these compounds, some b, y, a, and internal ions were observed and slight mass differences between the calculated and observed results are obtained. Their sequences were derived successfully. However, the MS/MS patterns of these analogs with Dphg and Lphg were very similar. It is hard to distinguish them by MS/MS spectra. Moreover, if the third position was substituted by phenylglycine （L or D）, a rearrangement could occur in MS/MS experiment to lose proline residue.

关 键 词：Endomorphin-2 Opioid peptide analogs PHENYLGLYCINE ESI-FT MS 

分 类 号：TQ46[化学工程—制药化工]