LKB1基因转染对人肺腺癌细胞生物学行为的影响  

Effect of transfection of LKB1 on biological behavior of human lung adenocarcinoma cells

作　　者：梁璇[1] 南克俊[1] 李春丽[1] 姚煜[1] 田涛[1] 王淑红[1] 

机构地区：[1]西安交通大学医学院第一附属医院肿瘤内科,陕西西安710061

出　　处：《细胞与分子免疫学杂志》2008年第12期1140-1142,1146,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金资助项目(30270590)

摘　　要：目的:探讨外源性LKB1基因表达对人肺腺癌细胞生物学行为的影响。方法:应用巢式PCR方法扩增LKB1基因编码区,利用脂质体转染技术将真核表达重组体pcDNA-LKB1质粒和空载体pcDNA3.1质粒分别导入A549细胞,经G418筛选后获得稳定转染细胞克隆,Western blot检测LKB1和STAT3在A549细胞中的表达变化。通过绘制生长曲线和克隆形成试验检测获得性表达LKB1基因后肺癌细胞的增殖变化,流式细胞术(FCM)分析其细胞周期及其凋亡的变化。结果:成功获得了稳定表达外源性LKB1基因的A549细胞系,与转染空白载体组比较,转染LKB1基因的细胞生长速度明显减慢,细胞周期中G1/G0期比例明显增加,S期比例减少,细胞凋亡率升高;同时STAT3蛋白的磷酸化水平明显降低。结论:LKB1可能通过下调STAT3蛋白磷酸化水平的表达从而抑制A549细胞的恶性生物学行为。AIM： To determine the effects of exogenous LKB1 gene on biological behavior of lung carcinoma cells. METHODS： Nest PCR was used to clone LKB1 gene pcD-NA-LKB1 and pcDNA3.1 were introduced into A549 cell line by lipofectin transfection, and the A549 cells stably expressing LKB1 gene were established by G418 selection. The expression of LKB1 and STAT3 protein was detected by Western blot. Cell proliferation was observed by growth curve and clone formation. Cell cycle and apoptosis were assayed by flow cytometry（FCM）. RESULTS： A549 cells stably expressing LKB1 protein were established. Compared with the vector transfected cells, the positive clone cells grew more slowly. FCM showed that more positive clone cells went into phase G0/G1 and fewer cells went into phase S. Moreover, there was significant difference on apoptosis between the two group cells. CONCLUSION： LKB1 protein might inhibit malignant biological behavior of A549 cells partly by downregulating the tyrosine-phosphorylated level of STAT3 protein.

关 键 词：LKB1 转染 增殖 凋亡 肿瘤 STAT3 

分 类 号：R734.2[医药卫生—肿瘤]