转聚磷激酶大肠杆菌诱导表达条件及磷浓度对其除磷能力的影响  

Effect of Induction Conditions and Phosphate Concentration on the Ability of Polyphosphate Kinase Gene Transferred E.coli to Remove Phosphate

作　　者：任晶[1] 肖琳[1] 郑小红[1] 杨柳燕[1] 

机构地区：[1]污染控制与资源化研究国家重点实验室南京大学环境学院,南京210093

出　　处：《环境保护科学》2008年第6期1-3,22,共4页Environmental Protection Science

基　　金：国家"973"项目(2002CB412307)资助

摘　　要：在优化转聚磷激酶基因的大肠杆菌(BL-PPK)诱导表达条件基础上探讨了外界磷浓度对其除磷能力的影响,发现37℃细胞对数生长早期添加1.5mmol/L IPTG时聚磷激酶的表达活性和细胞除磷效率最高,同时BL-PPK对高达35mg/L的磷酸盐在6.5h内的去除效果达到99%以上,并以聚磷的形式积累在细胞内。On the basis of optimizing the induction conditions for gene transferred E. coli （BL-PPK） which can overexpress polyphosphate kinase gene, this experiment studied the effect of environmental soluble phosphate on the ability of BL -PPK to remove pbosphate, and we got the conclusions： the optimal IPTG induction concentration for BL-PPK was 1.5mmol/L, the optimal initial cell density for induction was at the early log growth, the optimal induction temperature was 37℃. BL-PPK could remove above 99% of phosphates in 6. 5h when the environmental phosphate concentration was 35mg/L, with polyphosphate accumulated.

关 键 词：生物除磷 聚磷激酶 诱导条件 

分 类 号：X799.3[环境科学与工程—环境工程] Q786[生物学—分子生物学]