三嵌段高分子骨组织工程支架材料的表面多肽修饰与细胞粘附性研究  

The Surface Modification and the Cellular Adhesion Study of PLGA-[ASP-PEG]

作　　者：郝杰[1] 郑启新[2] 郭晓东[2] 吴永超[2] 宋玉林[2] 杨大志[2] 

机构地区：[1]重庆医科大学附属第一医院骨科,重庆400016 [2]华中科技大学同济医学院附属协和医院骨科,湖北武汉430022

出　　处：《生物骨科材料与临床研究》2008年第6期4-7,10,共5页Orthopaedic Biomechanics Materials and Clinical Study

基　　金：国家自然科学基金资助项目(30200063;30170270)

摘　　要：目的用甘氨酸-精氨酸-甘氨酸-天冬氨酸-丝氨酸-脯氨酸-半胱氨酸对三嵌段髙分子骨组织工程支架材料进行表面修饰,并检测其细胞粘附性。方法利用异双官能交联剂将甘氨酸-精氨酸-甘氨酸-天冬氨酸-丝氨酸-脯氨酸-半胱氨酸多肽固定在聚丙交酯/乙交酯/天冬氨酸-聚乙二醇材料表面,并进行X线光电子分光镜检测和表面接触角测定;体外培养骨髓基质细胞,接种至表面修饰的材料上,测定细胞粘附力,并和未修饰材料对比。结果固定交联剂和多肽后X线光电子分光镜检测示硫元素的含量分别为0.3%和0.2%;硫元素的结合能是164eV和163.9eV;表面接触角为60.2±2.364度;细胞粘附力为(521.45±134.98)×10-10牛顿。结论甘氨酸-精氨酸-甘氨酸-天冬氨酸-丝氨酸-脯氨酸-半胱氨酸能共价固定在聚丙交酯/乙交酯/天冬氨酸-聚乙二醇材料表面;多肽修饰后的材料能特异性的介导骨髓基质细胞粘附,增强其粘附力。Objective To modify the surface of poly（lactic acid/glycolic acid/asparagic acid-co-polyethylene glycol） （PLGA-[ASP-PEG]）, using GIy-Arg-Gly-Asp-Ser-Pro-Cys（GRGDSPC） peptide, and then test the cellular adhesion. Methods Use the heterobifunctional reagent Sulfo-LC-SPDP（Sulfosuccinimidy 16-[3＇-2-（pyridyldithio）-propionamido] hexanoate） to immobilize GRGDSPC peptide onto the surface of PLGA-[ASP-PEG], which was proved by XPS （X-ray photoelectron spectroscopy）. The contact angles were measured. Then marrow stromal cells （MSCs） were seeded onto the modified surfaces and the cell adhesive forces were measured. Compared the results with that of the PLGA-[ASP-PEG]. Results XPS analysis showed that sulfur element was 0.3% and 0.2% respectively, with sulfur＇s binding energy of 164eV and 163.9 eV, indicating GRGDSPC was immobilized on the surface. The contact angle was 60.2 degree, and the cellular adhesive force was （521.45±134.98）× 10^-10 Newton. Conclusion The GRGDSPC peptide was covalently immobilized on the surface of the PLGA-[ASP-PEG], which enhancing PLGA-[ASP-PEG]＇s adhesive force to the MSC.

关 键 词：骨 组织工程 支架材料 表面修饰 多肽 

分 类 号：R318.08[医药卫生—生物医学工程]