鞘内注射μ、δ阿片受体激动剂对大鼠骨癌痛的影响  

Effects of intrathecal μ opioid receptor and δ opioid receptor agonists on bone cancer pain in rats

作　　者：许爱军[1] 田玉科[1] 曹菲[1] 陈莎莎 高峰[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院麻醉学教研室,武汉市430030

出　　处：《实用医学杂志》2008年第23期4009-4011,共3页The Journal of Practical Medicine

基　　金：国家自然科学基金资助项目(编号:30672027)

摘　　要：目的:比较鞘内及RVM区注射μ阿片受体(MOR)和δ阿片变体(DOR)激动剂对大鼠骨癌痛及神经病理痛的痛行为学的影响,探讨MOR和DOR在骨癌痛发病机制中的作用。方法:雌性Wistar大鼠,分为14组:骨癌痛+NS组、骨癌痛+DAMGO(1、5、10μg)组、骨癌痛+DPDPE(0.1、0.5、1μg)组、SNI+NS组、SNI+DAMGO(0.5、1、5μg)组、SNI+DPDPE(0.1、0.5、1μg)组。注药20min后,观察不同剂量药物对大鼠触诱发痛及机械痛觉过敏反应的影响。结果:在骨癌痛大鼠,DAMGO5、10μg可显著升高大鼠双侧后爪对von fray的缩爪阈值,明显缩短术侧后爪对针刺的缩爪持续时间(P<0.05);DPDPE(0.1、0.5、1μg)对骨癌痛大鼠双侧后爪的触诱发痛和术侧后爪的机械痛觉过敏反应呈剂量依赖性的抑制作用;在SNI模型大鼠,DAMGO和DPDPE均呈剂量依赖性的抑制大鼠的触诱发痛和机械痛觉过敏反应(P<0.05)。结论:鞘内给予μ、δ阿片受体选择性激动剂对大鼠骨癌痛及神经病理性疼痛均有明显的镇痛作用,所需的剂量在骨癌痛大于神经病理性疼痛。Objective To compare the effects of intrathecal μ opioid receptor （MOR） and δ opioid receptor （DOR） agonists on bone cancer pain with that on neuropathic pain in rats and explore the roles of MOR and DOR in the pathogenesis of bone cancer pain （BCP）. Methods Female Wistar rats were divided to 14 groups： BCP/NS, BCP/DAMGO （1, 5, or 10 μg）, BCP/DPDPE （0.1, 0.5, or 1 μg）, SNI/NS, SNI/DAMGO （0.5, 1, or 5 μg）, SNI/DPDPE （0.1, 0.5, or 1 μg）. The effects of MOR and DOR agonists at different doses on mechanical allodynia and hyperalgesia were observed. Results In the rats with bone cancer pain, 5 or 10μg of intrathecal DAMGO significantly elevated the paw withdrawal threshold in the von Frey hair test on both hind paws and shortened the duration of puncture-induced paw withdrawal of the affected hide paw （P 〈 0.05）. 0.1, 0.5, or 1μg of DPDPE inhibited tactile allodynia in both hide paws and mechanical hyperalgesia in the affected hide paw in a dose-dependent fashion. In the SNI rats, both DAMGO and DPDPE inhibited mechanical allodynia and hyperalgesia in a dose-dependent manner （P 〈 0.05）. Conclusions Intrathecal MOR and DOR agonists can markedly relieve bone cancer pain and neuropathic pain in rats. The dosage of the agonists for caner pain is greater than that for neropathic pain.

关 键 词：骨肿瘤 疼痛μ阿片受体 Δ阿片受体 脊髓 

分 类 号：R738.1[医药卫生—肿瘤]