TNF-α对培养视网膜微血管内皮细胞与周细胞PDGF-B／PDGFR-β表达的作用  

Effects of TNF-α on the expression of PDGF-B and PDGFR-β of retinal microvessel endothelial cells and pericytes

作　　者：刘洪雷[1] 刘军[2] 张自峰[1] 周健[1] 王雨生[1] 惠延年[1] 

机构地区：[1]第四军医大学西京医院眼科,中国陕西省西安市710032 [2]第四军医大学唐都医院心脏内科,中国陕西省西安市710038

出　　处：《国际眼科杂志》2008年第12期2387-2390,共4页International Eye Science

基　　金：中国国家自然科学基金资助项目(No:30371516)~~

摘　　要：目的:研究TNF-α对内皮细胞与周细胞PDGF-B/PDGFR-β表达的作用。方法:常规剂量100μg/L以及大剂量2450μg/LTNF-α作用于培养的视网膜微血管内皮细胞、周细胞,通过免疫组化、Western Blot、RT-PCR检测PDGF-B/PDGFR-β表达。结果:内皮细胞组大剂量TNF-α组,PDGF-B表达显著下调,8h表达开始出现下调,在12h,PDGF-B的表达量为原来的39%;在人视网膜微血管周细胞组,与对照组相比,常规TNF-α剂量组PDGFR-β表达并未出现显著改变;大剂量TNF-α组,与对照组相比,PDGFR-β表达在12h下调为45%。结论:TNF-α可引起内皮细胞与周细胞PDGF-B/PDGFR-β表达降低。AIM： To investigate alterations of the gene expression of PDGF-B and PDGFR-β of bovine retinal microvessel endothelial cells and pericytes induced by TNF-α. METHODS： Retinal microvessle endothelial cells and pericytes were isolated and reversed transcriptase polymerase chain reaction （RT-PCR） and western blot analyses were performed to determine the mRNA and protein content for PDGF-B and PDGFR-β induced by different concentration of TNF-α. RESULTS： By semi-quantity RT-PCR, for the endothelial cells groups, the expressions of PDGF-B were downregulated about 39% at 12h in the large dosage of TNF-α group （2 450ug/L） ; For pericytes groups, the expression of PDGFR-β did not show significant changes in the routine dosage of TNF-α group （vs control ）, the expressions of PDGFR-β were down-regulated about 45% at 12h in the large dosage of TNF-α group. CONCLUSION： TNF-α, a typical factor of inflammation in the early stage of diabetic retinopathies, could cause the down-regulation of the expression of PDGF-B/PDGFR- β of endothelial cells and pericytes, which maybe a cause of the pericytes loss in the early stage of DRP.

关 键 词：内皮细胞 周细胞 TNF—α PDGF—B PDGFR—β 增生 

分 类 号：R774.1[医药卫生—眼科] R512.602[医药卫生—临床医学]