全反式视黄酸对体外培养人巩膜成纤维细胞的抑制作用  

All-trans retinoic acid inhibited human scleral fibroblasts proliferation and activity

作　　者：崔冬梅[1] 高岩[1] 任亚琳[1] 聂玉红 胡守龙[1] 李传旭[1] 曾骏文[1] 

机构地区：[1]中山大学中山眼科中心中山大学眼科学国家重点实验室,中国广东省广州市510060

出　　处：《国际眼科杂志》2008年第12期2397-2400,共4页International Eye Science

基　　金：中国国家自然科学基金资助项目(No.30572005)~~

摘　　要：目的:观察全反式视黄酸(all-trans retinoicacid,RA)对体外培养的人巩膜成纤维细胞(human scleral fibroblasts,HSF)的形态、增殖能力和自我更新能力以及细胞周期的影响,探讨RA对巩膜成纤维细胞的作用。方法:原代培养HSF,波形蛋白和角蛋白对细胞进行鉴定。用不同浓度RA培养HSF,相差显微镜下动态观察其形态和数量变化;MTT法检测HSF在不同浓度RA培养后的细胞增殖和自我更新能力;FCM方法检测不同浓度RA对HSF细胞周期的影响。结果:MTT法显示RA对HSF有明显的抑制作用,该效应呈剂量依赖性反应。RA培养后HSF细胞数目减少,细胞收缩,突起减少,部分裂解,胞浆内有空泡和颗粒形成,细胞之间的间隙增宽,细胞变得细长,呈束状或不规则排列。FCM结果显示和对照组比较,随着RA浓度和时间的增加,G0/G1期细胞的比例逐渐上升,而S期和G2期细胞比例相应下降,细胞被阻滞在G0/G1期。结论:RA可抑制体外培养HSF的增殖和DNA合成。AIM： To evaluate the effects of all-trans retinoic acid （RA） on the morphous, proliferation, activity and cell cycle of human scleral fibroblasts （HSF）. METHODS： Primary HSF was cultured in vitro. The dynamic morphous and number changes of HSF after cultured with different concentration RA were observed under light microscopy. MTT colorirnetric assay was used to investigate the effect of RA on proliferation and activity of HSF. Cell cycle and apoptosis of HSF were detected by flow cytometry analysis （FCM）. RESULTS： The number of HSF decreased and the cells shank, disrupted and slendered, the interspace of cells became wider after cultured with different concentration of RA. 10umol/L of RA could significantly inhibit the proliferation of HSF in a dose dependent and time dependent manner. The results of FCM analysis showed that percentage of HSF in G0/G1 phase was increased and that of S and G2/M phase were decreased when the cells were stimulated with RA in comparison with control group. CONCLUSION： RA inhibits HSF growth through decreasing the proliferation and DNA synthesis of HSF in vitro.

关 键 词：视黄酸 巩膜 成纤维细胞 

分 类 号：R773.1[医药卫生—眼科]