左旋卡尼汀对大鼠缺血心肌细胞凋亡相关蛋白表达的影响  

Effect of L-carnitine on Expression of Cardiomyocyte Apoptosis-related Protein in Rat Model of Myocardial Ischemia

作　　者：荆翠平[1,2] 陈丽娟[1] 刘磊[1] 于景文 

机构地区：[1]吉林大学第二临床医院心内科,长春130041 [2]解放军二0八医院神经介入科,长春130062 [3]白城市通榆县医院心内科,白城137000

出　　处：《中国生物制品学杂志》2008年第12期1098-1100,共3页Chinese Journal of Biologicals

基　　金：长春市科技厅资助课题(06GG31)

摘　　要：目的观察左旋卡尼汀对大鼠缺血心肌细胞凋亡相关蛋白Bcl-2和Caspase-3表达的影响。方法将SD大鼠随机分成3组:空白对照组、缺血组和左旋卡尼汀组。缺血组及左旋卡尼汀组给予异丙肾上腺素建立心肌缺血模型,左旋卡尼汀组提前经腹腔注射左旋卡尼汀。原位末端标记(TUNEL)法测定凋亡的心肌细胞,免疫组化法测定Bcl-2及Caspase-3蛋白的表达。结果左旋卡尼汀组与缺血组相比,心肌组织Bcl-2蛋白表达率显著升高,细胞凋亡率和Caspase-3蛋白表达率显著降低。结论左旋卡尼汀在异丙肾上腺素所致的心肌缺血大鼠模型中,对缺血缺氧心肌有保护作用,其机制可能是通过调节Bcl-2和Cas-pase-3介导的细胞凋亡而实现的。Objective To observe the effect of L-carnitine on expressions of rat cardiomyocyte apoptosis-related proteins Bcl-2 and Caspase-3. Methods Fifty SD rats were divided into 3 groups, i. e. control, model and L-carnitine groups randomly. The rats in model group were treated with isoproterenol, and those in L-carnitine group with L-carnitine prior to isoproterenol, while those in control group with physiological saline. The apoptotic cardiomyocytes were determined by TUNEL method, and the expressions of Bcl- 2 and Caspase-3 proteins by immunohistochemical method. Results Compared with those in model group, the expression rates of Bcl-2 protein in cardiac muscle tissues of rats in L-carnitine group increased significantly, while the cardiomyocyte apoptosis rate and Caspase-3 protein expression rate decreased significantly. Conclusion L-carnitine may protect the myocardium of rats with isoproterenol-induced myocardial ischemia by a potential mechanism of modulating the cardiomyocyte apoptosis mediated by Bcl-2 and Caspase-3.

关 键 词：左旋卡尼汀 心肌细胞 心肌缺血 凋亡 BCL-2蛋白 CASPASE-3蛋白 

分 类 号：R363[医药卫生—病理学] R542.2[医药卫生—基础医学]