酪丝缬肽对人肝癌细胞SMMC-7721血管生成相关基因表达谱的影响  

Microarray analysis of tyroservatide-regulated angiogenesis gene expression profile in human hepatocellular carcinoma cell SMMC-7721

作　　者：张宁[1] 王鲁[1] 赵一鸣[1] 梁英[1] 吴伟忠[1] 樊嘉[1] 汤钊猷[1] 

机构地区：[1]复旦大学肝癌研究所,上海市200032

出　　处：《世界华人消化杂志》2008年第33期3719-3723,共5页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.30571800;高等学校全国优秀博士论文作者专项基金资助项目;No.200263~~

摘　　要：目的:观察小分子三肽化合物酪丝缬肽(tyroservatide,YSV)对人肝癌细胞系SMMC-7721的抑制作用,并分析YSV对肿瘤细胞血管生成相关基因表达的影响.方法:建立人肝癌细胞SMMC-7721的体外培养体系,采用四甲基偶氮唑蓝(MTT)比色法检测YSV对体外培养的SMMC-7721的增殖抑制作用,应用功能分类基因芯片分析人肝癌细胞SMMC-7721经药物作用后血管生成相关基因表达的差异变化,同时应用RT-PCR方法验证相关基因的表达.结果:YSV能显著抑制人肝癌SMMC-7721的生长,10mg/LYSV作用72h对SMMC-7721的增殖抑制率为42.34%,与对照组比较有统计学意义(P<0.05).在113个血管生成相关基因中,YSV组相比对照组,基因下调0.667倍以上的有7个.RT-PCR在基因水平上证实YSV能显著抑制血管内皮生长因子(VEGF)和白介素8(IL-8)的表达.结论:YSV在体外能够显著抑制人肝癌细胞SMMC-7721的生长,并能在基因水平上下调肿瘤细胞血管生成相关因子的表达.AIM：To observe inhibitory effects of tripeptide compound tyroservatide（YSV）on proliferation of human hepatocarcinoma cell SMMC-7721,and to apply cDNA microarray to analyze its influence on angiogenesis-related gene expression profile of SMMC-7721.METHODS：a culture system of SMMC-7721 in vitro was established.The inhibitory effects of tyroservatide on growth of SMMC-7721 was detected using 3-（4,5-Dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide（MTT）colorimet-ric assay.Gene expression profile of SMMC-7721 was detected by Oligo angiogenesis microarray analysis.Semi-quantitative reverse transcription-polymerase chain reaction（RT-PCR）was used to verify the results of the specific genes.RESULTS：YSV significantly inhibited the growth of SMMC-7721;the proliferative inhibi-tion rate was 42.34%,when YSV was used for 72 hours at concentration of 10 mg/L.The differ-ence was significant compared with the control group（P〈0.05）.After YSV treatment,7 genes were down-regulated at least 0.667 fold among a total of 113 angiogenesis genes,compared with control group.YSV significantly inhibited the gene expression of angiogenesis factors such as vascular endothelial growth factor（VEGF）and interleukin-8（IL-8）confirmed by RT-PCR.CONCLUSION：YSV could significantly in-hibit the growth of human hepatocarcinoma SMMC-7721 in vitro,and down-regulate expres-sion of angiogenesis factors at genetic level.

关 键 词：酪丝缬肽 CDNA微阵列 SMMC-7721 血管内皮生长因子 白介素8 

分 类 号：R735.7[医药卫生—肿瘤]