中国荷斯坦牛DGAT1基因与产奶性状关联分析  

Association Analysis between DGAT1 Gene and Milk Production Traits in Chinese Holstein

作　　者：贾晋[1,2] 马妍[1] 孙东晓[1] 张毅[1] 张沅[1] 

机构地区：[1]中国农业大学动物科技学院,北京100193 [2]内蒙古师范大学附属中学,呼和浩特010020

出　　处：《畜牧兽医学报》2008年第12期1661-1664,共4页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：“948”项目(2006-G48);国家科技支撑计划(2006BAD04A01);“863”项目(2007AA10Z157);“973”项目(2006CB102107)资助

摘　　要：以北京地区来自17个公牛家系的1 222头中国荷斯坦母牛为试验材料,采用PCR-RFLP技术对DGAT1基因进行了遗传多态性分析,共检测到KK、KA和AA 3种基因型,频率分别为0.143 2、0.609 7和0.247 1;采用混合动物模型对数据进行拟合,通过SAS(8.02)软件对5个产奶性状与DGAT1基因的关联程度进行了统计分析。结果表明,DGAT1不同基因型间的305 d产奶量、乳脂量、乳蛋白量差异极显著(P<0.01);多重比较结果显示:AA基因型的305 d产奶量和乳蛋白量显著高于KK基因型(P<0.01),其乳脂量显著低于KK基因型(P<0.01)。结果提示DGAT1基因对奶牛产奶性状具有较大遗传效应,可以用于中国荷斯坦牛产奶性状的分子标记辅助选择。With PCR-RFLP technique, the polymorphism in the exon8 of DGAT1 gene were investigated in a population of 1 222 Chinese Holstein cows from 17 bull families. Three kinds of genotypes were found and the genotypic frequencies of KK, KA and AA were 0. 143 2, 0. 609 7 and 0. 247 1, respectively. Using SAS （8.02） software with animal model, significant associations between the K232A mutation within exon8 of DGAT1 gene and three milk production traits were revealed. Duncan＇s multiple-range test showed that the cows with AA genotype had higher 305- day milk yield and protein yield than those with KA and KK genotypes （P〈0.01）, while had lower fat yield （P〈0. 01）. The results suggested that DGAT1 gene has a great effect on milk production traits and could be a useful genetic marker in Chinese Holstein breeding.

关 键 词：中国荷斯坦牛 DGAT1 产奶性状 关联分析 PCR—RFLP 

分 类 号：S823.91[农业科学—畜牧学] S823.2[农业科学—畜牧兽医]