贲门癌、胃癌患者红细胞C3b受体与CD4^+/CD8^+比值、NK细胞活性的相关性研究  

Red Blood C3b Receptor and Ratio of CD4^+/CD8^+ and NK Cell Activity in Gastric Cardia Carcinoma and Gastric Cancer Patients

作　　者：梁索源[1] 单保恩[1] 白引苗[1] 武立华[1] 艾军[1] 

机构地区：[1]河北医科大学第四医院肿瘤所,河北石家庄050011

出　　处：《癌变．畸变．突变》2008年第6期456-458,共3页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：河北省高校强势特色学科肿瘤学科项目资助

摘　　要：背景与目的:研究贲门癌、胃癌患者红细胞C3b受体(RBC-C3bR)与CD4+/CD8+比值、NK细胞活性的相关性。材料与方法:利用酵母菌花环试验、克隆抗体致敏花环法和MTT法对53例贲门癌、51例胃癌患者及30例正常人外周血RBC-C3bR阳性率、T细胞CD3+、CD4+百分率、CD4+/CD8+比值及自然杀伤细胞(NK)活性进行测定。结果:贲门癌组、胃癌组RBC-C3bR阳性率、CD3+、CD4+细胞百分率、CD4+/CD8+比值和NK细胞杀伤活性均比正常对照组低,差异具有统计学意义(F=28.68,P<0.01),而贲门癌与胃癌组间比较,差异无统计学意义(t=0.081～0.871,P均>0.05);经统计学相关性分析显示,贲门癌、胃癌组RBC-C3bR阳性率与CD4+/CD8+比值、NK细胞杀伤活性间呈正相关(贲门癌RBC-C3bR阳性率与CD4+/CD8+比值:r=0.431,P<0.01;RBC-C3bR阳性率与NK细胞活性:r=0.347,P<0.05;胃癌RBC-C3bR阳性率与CD4+/CD8+比值:r=0.429,P<0.01;RBC-C3bR阳性率与NK细胞活性:r=0.528,P<0.01)。结论:贲门癌、胃癌患者红细胞免疫与白细胞免疫功能均受到抑制。BACKGROUND AND AIM： To investigate the correlation between red blood C3b receptor （RBC-C3bR） and lymphocytic cell （TC） immune function in tumor patients. MATERIALS AND METHODS： RBC-C3bR, natural killer （NK） cell activity and T lymphocyte subsets of 53 patients with gastric cardia carcinoma, 51 gastric cancer and 30 healthy controls were measured by microzyme garland test , MTT assay and monoclonal anti-human T lymphocyte antibody. RESULTS： Compared with the control group, RBC-C3bR , NK cell activity, CD3 ^＋, CD4^＋, CD4^＋/ CD8^＋ ratio were significantly decreased in tumor patients （F = 28.68 , P 〈 0.01）. Positive correlations were observed between RBC-C3bR with both NK cell activity and CD4^＋/ CD8^＋ ratio （gastric cardia carcinoma RBC-C3bR and CD4＋/ CD8＋ ratio： r= 0.431, P 〈 0.01; RBC-C3bR and NK cell activity： r = 0.347, P 〈 0.05; gastric cancer RBC-C3bR and CD4^＋/CD8 ^＋ ratio： r = 0.429, P 〈 0.01; RBC-C3bR and NK cell activity,r = 0.528, P 〈 0.01）. CONCLUSION： The immune funtion of erythrocytes was inhibited same as lymphocytes and there was close relationship between them in gastric cardia carcinoma, gastric cancer patients.

关 键 词：贲门癌 胃癌 红细胞C3B受体 淋巴细胞亚群 NK细胞 

分 类 号：R735.2[医药卫生—肿瘤]