错配修复基因的异常表达与淮安食管癌发病的关系  

Association between Mismatch Repair Gene Expression and Esophageal Squamous Cell Carcinomas in Huaian

作　　者：刘冉[1] 尹立红[1] 浦跃朴[1] 王仪[2] 潘恩春[2] 

机构地区：[1]东南大学公共卫生学院,南京210009 [2]江苏省淮安市疾病预防控制中心,江苏淮安223001

出　　处：《癌变．畸变．突变》2008年第6期467-469,474,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家自然科学基金资助项目(30800891;30671732;30571540);江苏省预防医学基金项目(Y2006007)

摘　　要：背景与目的:研究错配修复相关基因hMLH1和hMSH2的mRNA表达水平与食管癌发病的关系。材料与方法:收集112例淮安地区食管癌新发病例的食管癌组织及远端癌旁组织,采用实时荧光定量PCR方法定量分析食管癌组织与癌旁组织中hMLH1和hMSH2基因的表达水平,应用t检验和logistic回归分析评价基因的表达水平与食管癌的关系。结果:hMSH1基因的mRNA表达水平在食管癌与癌旁组织间的差异不明显,而hMSH2基因的mRNA表达水平在食管癌组织显著低于癌旁组织(下调了51.2),条件logistic回归分析表明hMSH2的表达下调与食管组织癌变有统计学关联(OR=1.311,95CI:1.075～1.599)。结论:错配修复相关基因hMSH2的表达水平下降提示食管癌组织错配修复能力异常,可能是淮安地区食管癌发病的危险因素之一。BACKGROUND AND AIM： To determine whether the mRNA expressions of hMLH1 and hMSH2 genes involved in mismatch repair are associated with the risk of ESCC. MATERIALS AND METHODS： 112 patients with newly diagnosed, untreated ESCC were recruited in Huai＇ an county. Esophageal cancer tissues and tissues adjacent to the tumors were collected to determine the mRNA levels of hMLH1 and hMSH2 genes by quantitative real-time reverse transcription-PCR using SYBR Green I with β-actin as an internal control, t-test and logistical regression analysis were used to test the differences of gene expression between cancer tissues and tissues adjacent to the tumors. RESULTS： Compared with tissues adjacent to the tumors, the relative expression level of hMSH2 was significantly lower in esophageal cancer tissues （P = 0.010, t-test）. The conditional logistic regression analysis showed that the increased risk for esophageal cancer was significantly associated with decreased expression of hMSH2 （OR = 1.311, 95% CI： 1.075- 1.599） . CONCLUSION： Reduced expression of hMSH2 was significantly associated with risk for ESCC, suggesting this might be important for the early progression of ESCC in Huaian of China.

关 键 词：错配修复基因 基因表达 实时荧光定量PCR 食管癌 

分 类 号：R730.45[医药卫生—肿瘤]